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| 木荷1代育种群体遗传多样性分析 | |
| 引用本文： | 辛娜娜,张蕊,徐肇友,肖纪军,王帮顺,周志春.木荷1代育种群体遗传多样性分析[J].林业科学研究,2015,28(3):332-338. |
| 作者姓名： | 辛娜娜 张蕊 徐肇友 肖纪军 王帮顺 周志春 |
| 作者单位： | 1. 中国林业科学研究院亚热带林业研究所,浙江省林木育种技术研究重点实验室,浙江杭州311400;华中农业大学园艺林学学院,湖北武汉430070 2. 中国林业科学研究院亚热带林业研究所,浙江省林木育种技术研究重点实验室,浙江杭州311400 3. 浙江省龙泉市林业科学研究院,浙江龙泉,323700 |
| 基金项目： | "十二·五"国家科技支撑计划课题(2012BAD01B04);林业公益性行业科研专项重大项目(201104001);浙江省农业新品种选育重大科技专项竹木育种协作组重点项目(2012C12908-6);福建省林木种苗科技攻关四期项目木荷课题 |
| 摘 要： | 木荷为我国亚热带地区主要的珍贵优质阔叶用材树种和生态防护树种.利用筛选的14对多态性强的SSR引物,对木荷1代育种群体中来自15个产地133个亲本进行遗传多样性分析,为其优异种质资源保存、杂交亲本选配及新种质创制提供科学依据.结果表明:14对引物共扩增86个位点,每对引物检测到的等位基因数(Na)变异范围为2～11个,平均等位基因数(Na)为6.14个,平均有效等位基因数(Ne)为3.23个,平均观察杂合度(Ho)为0.572 0,平均Shannon信息指数(I)和平均Nei's基因多样性指数(Nei)分别为1.224 7和0.599 0,说明木荷1代育种群体具有丰富的遗传多样性,其中,福建建瓯产地遗传多样性最高,浙江遂昌产地遗传多样性最低.木荷1代育种群体中成对亲本间遗传距离为0.023 3～1.633 8,平均为0.6067.不同产地遗传多样性与纬度呈显著的负相关关系(r=-0.5162,p=0.048 9).通过UPGMA聚类,可将133个育种亲本分成3个类群,其中,类群3又分为4个亚类群.木荷亲本选配时,应充分考虑优树的产地来源. |
| 关 键 词： | 木荷 育种亲本 遗传多样性 遗传距离 SSR |
| 收稿时间： | 2014/10/9 0:00:00 |
Genetic Diversity among Breeding Parents of Schima superba Revealed by SSR |
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| XIN Na-n,ZHANG Rui,XU Zhao-you,XIAO Ji-jun,WANG Bang-shun and ZHOU Zhi-chun.Genetic Diversity among Breeding Parents of Schima superba Revealed by SSR[J].Forest Research,2015,28(3):332-338. | |
| Authors: | XIN Na-n ZHANG Rui XU Zhao-you XIAO Ji-jun WANG Bang-shun and ZHOU Zhi-chun |
| Institution: | Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Zhejiang Provincial Key Laboratory of TreeBreeding, Hangzhou 311400, Zhejiang, China;Gardening Forestry Institute of Huazhong Agricultural University, Wuhan 430070, Hubei, China;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Zhejiang Provincial Key Laboratory of TreeBreeding, Hangzhou 311400, Zhejiang, China;Research Institute of Longquan City, Zhejiang Province, Longquan 323700, Zhejiang, China;Research Institute of Longquan City, Zhejiang Province, Longquan 323700, Zhejiang, China;Research Institute of Longquan City, Zhejiang Province, Longquan 323700, Zhejiang, China;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Zhejiang Provincial Key Laboratory of TreeBreeding, Hangzhou 311400, Zhejiang, China |
| Abstract: | Schima superba is a representative, widely distributed species of broadleaf tree found in the subtropical forests of southern China. It valued commercially for its timber and ecology using. We used SSR molecular technique to investigate the genetic diversity and structure of S. superba, which would formulate the breeding strategy and provide insight for selecting breeding parents. 14 pairs of SSR primers were selected and 133 individuals came from 15 provenances were conducted in this experiment. The results showed that 86 polymorphic loci with alleles ranged from 2 to 11 were detected. The observed number of alleles, number of effect alleles, observed heterozygosity, Shannon information index and Nei's gene diversity were 6.14, 3.23, 0.572 0, 1.224 7 and 0.599 0 respectively. The breeding parents of S. superba held higher genetic diversity and the genetic diversity in Jian'ou of Fujian Province was highest, but it was lowest in Suichang of Zhejiang Province. The genetic difference was large among the individuals of the breeding parents of S. superba. The genetic distance was ranged from 0.023 3 to 1.633 8 with the average value of 0.606 7. Shannon's index of breeding parents was significantly negative with the latitudes of the seed sources (r=-0.516 2, p=0.048 9). The UPGMA cluster analysis showed that 133 individuals clustered into three groups and the third group could be divided into 4 sub-groups. It is important to consider the origin of the trees when selected the parents for hybridization. |
| Keywords: | Schima superba breeding parents genetic diversity genetic identity simple sequence repeat |
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