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| 转录因子CBF基因转化烟草抗寒性指标的检测 | |
| 引用本文： | 沙丽娜,郭兆奎,万秀清,程红梅.转录因子CBF基因转化烟草抗寒性指标的检测[J].中国林副特产,2009(1):4-7. |
| 作者姓名： | 沙丽娜 郭兆奎 万秀清 程红梅 |
| 作者单位： | 1. 黑龙江八一农垦大学植物科技学院,黑龙江,大庆,163319 2. 黑龙江八一农垦大学植物科技学院,黑龙江,大庆,163319;黑龙江省烟草科学研究所,牡丹江,157011 3. 黑龙江省烟草科学研究所,牡丹江,157011 4. 中国农业科学院生物技术研究所 |
| 基金项目： | 国家烟草专卖局科技计划 |
| 摘 要： | 为了解棉花转录因子CBF基因转化烟草的抗寒性，将苗龄21d的转基因及其野生型T2代烟苗进行0、-1、-2、-3、-4℃低温处理4h，分别进行各处理材料的电解质渗透率、丙二醛含量和可溶性糖含量的检测。结果表明转基因烟草的电导率和丙二醛含量均低于非转基因烟草，而转基因烟草的可溶性糖含量高于非转基因烟草。证实转CBF基因可以提高烟株的抗寒性。 |
| 关 键 词： | 转基因烟草 CBF基因 抗寒性 |
Test of the Cold-resistance Indicators of the CBF Genetically Modified Tobacco |
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| Sha Lina,Guo Zhaokui,Wan Xiuqing,Cheng Hongmei.Test of the Cold-resistance Indicators of the CBF Genetically Modified Tobacco[J].Forest By-product and Speciality in China,2009(1):4-7. | |
| Authors: | Sha Lina Guo Zhaokui Wan Xiuqing Cheng Hongmei |
| Institution: | 1.Heilongjiang August First Land reclamation university;Daqing 163319;2.Heilongjiang Tobacco Research Institute;Mudanjiang 157011;3.Chinese Academy of Agricultural Sciences Biotechnology Research Institute |
| Abstract: | In order to know stress tolerance of tobacco, T2 generation transgenic （CBF gene in Gossypium hirsutum） and non- transgenic tobacco was treated with different low temperature （0,-1 ,-2 ,-3 ,-4℃ ）. The conductivity,the concentration of propyl and sugar were tested. The results indicated that the conductivity and the concentration of propyl of transgenic tobacco were lower than non-transgenic tobacco. However, the concentration of sugar of transgenic tobacco was higher than non-transgenic tobacco. These results demonstrated that the cold tolerance of transgenic tobacco level was enhanced. |
| Keywords: | Transgenic tobacco CBF gene Cold tolerance |
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