| 构树染色体制片效果影响因素与核型分析 |
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| 引用本文： | 闫东方,周玮,王鑫,任颖,杨恩点,陈晓阳.构树染色体制片效果影响因素与核型分析[J].浙江林业科技,2019(3):37-44. |
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| 作者姓名： | 闫东方 周玮 王鑫 任颖 杨恩点 陈晓阳 |
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| 作者单位： | 广东省木本饲料工程技术中心;华南农业大学林学与风景园林学院;亚热带农业生物资源保护与利用国家重点实验室;广东省森林植物种质创新与利用重点实验室 |
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| 基金项目： | 华南地区主要木本饲料植物种质资源创新利用关键技术研究与示范(2017KJCX029) |
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| 摘 要： | 于2017年10月采集广东省广州市的构树Broussonetia papyrifea种子5 000粒,2018年2月进行种子萌发,通过常规压片方法进行核型分析。结果表明,在2018年3月2日至5月9日的10:00-10:30,发育7 d的材料有丝分裂指数最高,相比于化学处理方法,用冰水混合物预处理12h时染色体形态更清晰易辨;解离10min时根尖细胞染色体分散较好。构树染色体数目为26条,为二倍体,核型公式为2 n=2 x=26 m,核型属于2A型。
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| 关 键 词： | 构树 染色体 制片技术 核型分析 |
Influencing Factors of Tabletting Chromosome of Broussonetia papyrifea and Karyotype Analysis |
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| YAN Dong-fang,ZHOU Wei,WANG Xin,REN Ying,YANG En-dian,CHEN Xiao-yang.Influencing Factors of Tabletting Chromosome of Broussonetia papyrifea and Karyotype Analysis[J].Journal of Zhejiang Forestry Science and Technology,2019(3):37-44. |
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| Authors: | YAN Dong-fang ZHOU Wei WANG Xin REN Ying YANG En-dian CHEN Xiao-yang |
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| Institution: | (Guangdong Woody Forage Engineering Center, Guangzhou 510642, China;College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou 510642, China;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China) |
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| Abstract: | In October of 2017, 5000 seeds were collected from Broussonetia papyrifea in Guangzhou, Guangdong province, and in February of 2018, germination breaking was carried out. Caryotype analysis was implemented by pellet method. The result showed that the highest mitotic index was obtained from 7-day radicals at 10:00 and 10:30 during March 2 to May 9 of 2018. The morphology of chromosome was clearer when the radicals were treated with ice water for 12 hours. The dispersion of chromosomes was the best when 10 minutes was dissociated. The chromosome numbers of B. papyrifera were 26, diploid, and the karyotype formula was 2 n=2 x=26 m, and the karyotype belonged to 2 A type. |
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| Keywords: | Broussonetia papyrifea chromosome pellet method karyotype analysis |
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