Supercritical fluid extraction of sapogenins from tubers of Smilax china |
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Authors: | Shu Xiao-shun Gao Zhong-hong Yang Xiang-liang |
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Institution: | Institute of Materia Medica, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. |
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Abstract: | Supercritical CO(2) fluid extraction (SFE-CO(2)) was used to extract the sapogenins after acid hydrolysis from Smilax china tubers. The influence of extraction variables, such as modifier, pressure, temperature and extraction time, were studied. SFE-CO(2) was found to produce higher yield than conventional solvent extraction. The highest yield (0.454%) of sapogenins, mostly containing diosgenin, was obtained using 35 MPa pressure, 65 degrees C and 95% EtOH as a modifier for 180 min, higher than that obtained with conventional extraction methods (0.385%). |
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Keywords: | Supercritical CO2 fluid extraction (SFE-CO2) Smilax china Sapogenins |
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