| 杂交鹅掌楸体细胞胚萌发培养的研究 |
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| 引用本文： | 程强强,戴小英,宋晓琛,江香梅,肖复明.杂交鹅掌楸体细胞胚萌发培养的研究[J].江西林业科技,2014(5):1-3. |
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| 作者姓名： | 程强强 戴小英 宋晓琛 江香梅 肖复明 |
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| 作者单位： | 江西省林业科学院·江西省植物生物技术重点实验室，江西 南昌 330013 |
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| 基金项目： | 江西省科技计划项目“杂交马褂木体培苗繁育技术研究”，国家林业局科技推广示范项目2012[50]号部分内容。 |
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| 摘 要： | 以杂交鹅掌楸体细胞胚为材料,研究体胚不同发育阶段、大量元素和维生素C、基因型、激素组合以及移栽基质对体胚苗萌发和移栽存活的影响。结果表明：鱼雷型胚萌发效果最好;3/4 MS培养基较适合体胚苗的萌发;Vc有利用保持体胚苗维持正常形态。不同基因型体胚苗萌发差异较大,基因型1×5002萌发效果最好,成苗率为84.26%;而1×4088仅有13.24%。KT 0.1 mg/L＋IBA 0.1 mg/L有利于促进体细胞胚的萌发。不同基因型体胚苗移栽存活率有差异,黄心土较混合泥炭土更适合体胚苗移栽。
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| 关 键 词： | 杂交鹅掌楸 体细胞胚 萌发 |
Study on Germination of Somatic Embryos of Liriodendrom Hybrids |
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| CHENG Qiang-qiang,DAI Xiao-ying,SONG Xiao-chen,JIANG Xiang-mei,XIAO Fu-ming.Study on Germination of Somatic Embryos of Liriodendrom Hybrids[J].Jiangxi Forestry Science and Technology,2014(5):1-3. |
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| Authors: | CHENG Qiang-qiang DAI Xiao-ying SONG Xiao-chen JIANG Xiang-mei XIAO Fu-ming |
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| Institution: | (Jiangxi Provincial Key Lab for Plant Biotechnology, Jiangxi Academy of Forestry, Nanchang Jiangxi 330013, China) |
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| Abstract: | The influences of development period of somatic embryos, major element and vitamin C, genotypes, different combinations with plant growth regulators and transplant medium on germination and transplanting survival for somatic embryos of Liriodendrom hybrids were studied. The results showed that the optimal developement phase of germination was torpedo-shaped embryos, and the base cultural medium of 3/4 MS and with the vitamin C used of keeping normal form from somatic embryos. The somatic embryo germination among different genotypes at the same cultural condition was different significantly, for example, the genotype 1×5002 was the highest, up to 82.46%, the genotype 1×4088 just was to 13.24%. The medium KT 0.1 mg/L ＋ IBA 0.1mg/L can help to promote the germination of somatic embryogenesis. There were differences of seedling transplanting survival rate between the yellow subsoil and mixed peat soil, and the former was more suitable for transplanting than the later. |
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| Keywords: | Liriodendrom hybrids somatic embryos germination |
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