| 紫花含笑组培苗生根解剖机理研究 |
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| 引用本文： | 宋晓琛,程强强,戴小英,江香梅,肖复明.紫花含笑组培苗生根解剖机理研究[J].江西林业科技,2014(3):1-4,8. |
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| 作者姓名： | 宋晓琛 程强强 戴小英 江香梅 肖复明 |
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| 作者单位： | 江西省林业科学院·江西省植物生物技术重点实验室,江西南昌330013 |
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| 基金项目： | 农业科技成果转化资金项目“紫花含笑优良无性系‘荷瓣墨紫含笑’等无形繁育（2013GB2C500248）”和江西省科技计划项目“紫花含笑优良无性系‘荷瓣墨紫’等高效繁育技术推广（20133ACI90008）”。 |
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| 摘 要： | 应用光学显微技术对紫花含笑组培苗生根过程进行解剖学观察，结果表明：紫花含笑增殖苗在诱导生根前，没有根原始体，说明其生根类型为诱导生根型；在生根培养基中诱导生根时，发现根原基由髓射线与形成层交接处的薄壁细胞分化而成；生根过程中诱导产生的愈伤组织的存在，对根原基的形成有一定刺激作用，同时为后期不定根的生长提供营养，因此认为，适当诱导出愈伤组织，对紫花含笑不定根的生成起到重要的促进作用。本研究为紫花含笑组培苗生根机理提供了解剖学依据。
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| 关 键 词： | 紫花含笑 组培 生根 微解剖 机理 |
Rooting Mechanism Research of Tissue Culture Based on Micro-Anatomy in Michelia crassipes |
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| SONG Xiao-chen,CHENG Qiang-qiang,DAI Xiao-ying,JIANG Xiang-mei,XIAO Fu-ming.Rooting Mechanism Research of Tissue Culture Based on Micro-Anatomy in Michelia crassipes[J].Jiangxi Forestry Science and Technology,2014(3):1-4,8. |
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| Authors: | SONG Xiao-chen CHENG Qiang-qiang DAI Xiao-ying JIANG Xiang-mei XIAO Fu-ming |
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| Institution: | (Jiangxi Provincial Key Lab for Plant Biotechnology, Jiangxi Academy of Forestry, Nanchang Jiangxi 330013, China) |
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| Abstract: | The rooting mechanism of tissue culture was observed with the method of micro-anatomy in Michelia crassipes. The results showed that there was no root primordium before root-induced, and the rooting primordial cell was originated from the pith ray after induction. The callus can stimulate the formation of rooting primordium, and can provide the nutrients for root growth. It means that the callus played an important role in the root formation process. This study provided anatomical basis for tissue culture in M. crassipes. |
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| Keywords: | Michelia crassipes tissue culture rooting micro-anatomy mechanism |
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