| 抗生素对火炬松的器官发生植株再生的影响(英文) |
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| 作者姓名： | Latoya Harris Ronald J.Newton |
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| 作者单位： | Department of Biology，Howell Science Complex，East Carolina University，Greenville，Department of Biology，Howell Science Complex，East Carolina University，Greenville NC27858-4353，USA，NC27858-4353，USA |
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| 摘 要： | 本文研究了氨苄青霉素、羧苄青霉素和头孢霉素等3种抗生素对火炬松愈伤组织的生长和分化及不定芽生根的影响。结果表明,头孢霉素最有利于愈伤组织的诱导和生长,羧苄青霉素最有利于芽的分化,氨苄青霉素降低了不定芽的生根频率。所有试验的3种抗生素提高愈伤组织的形成和芽再生,但降低了芽的生根频率。这些结果表明,选择合适的抗生素对优化火炬松遗传转化体系有重要作用。图3表4参25。
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| 关 键 词： | 抗生素 火炬松 生根力 芽再生 |
Influences of antibiotics on plantlet regeneration via organogenesis in loblolly pine (Pinus taeda L.) |
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| Latoya Harris,Ronald J.Newton.Influences of antibiotics on plantlet regeneration via organogenesis in loblolly pine (Pinus taeda L.)[J].Journal of Forestry Research,2003,14(3). |
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| Authors: | TANG Wei Latoya Harris Ronald JNewton |
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| Abstract: | Three antibiotics ampicillin, carbenicillin, and cefotaxime were evaluated for their effects on induction, growth, and differentiation of organogenic calli, as well as rooting of regenerated shoots of three loblolly pine (Pinus taeda L.) genotypes. Of the antibiotics administered, cefotaxime maximally increased the frequency of callus formation and growth rate of organogenic calli, carbenicillin maximally increased the frequency of shoot regeneration and the average number of adventitious shoots per piece of organogenic callus, ampicillin maximally decreased the rooting frequency of regenerated shoots and mean number of roots per regenerated shoot, in comparison with antibiotic-free media. Compared with the control, ampicillin minimally increased the frequency of callus formation, cefotaxime minimally increased the frequency of shoot regeneration, and carbenicillin mini-mally decreased the rooting frequency of regenerated shoots in three loblolly pine genotypes tested. All three antibiotics in-creased the frequencies of callus formation and shoot regeneration, and reduced the rooting frequency of regenerated shoots suggested that the establishment of an efficient Agrobacterium tumefaciens-mediated transformation protocol for stable integra-tion of foreign genes into loblolly pine need to select a suitable antibiotic. This investigation could be useful for optimizing genetic transformation of conifers. |
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| Keywords: | Antibiotic Pinus taeda L Rooting ability Shoot regeneration |
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