| 快速测定黄姜中薯蓣皂甙元含量方法的研究 |
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| 引用本文： | 易自力,董静洲,蒋建雄.快速测定黄姜中薯蓣皂甙元含量方法的研究[J].西部林业科学,2005,34(1):57-59. |
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| 作者姓名： | 易自力 董静洲 蒋建雄 |
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| 作者单位： | 湖南农业大学细胞工程重点实验室,湖南,长沙,410128 |
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| 摘 要： | 经研究将测定黄姜中薯蓣皂甙元含量的方法改进为微量浸提分光光度法。此法采用微量样品以无水酒精浸提代替传统的回流抽提法, 以香兰素-浓硫酸作显色剂, 冰醋酸作溶剂, 在460NM处测定薯蓣皂甙元的含量。与传统的重量法、抽提分光光度法相比较, 此法测定方法简便, 结果准确, 成本低, 效率高。
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| 关 键 词： | 黄姜 薯蓣皂甙元 快速测定 |
| 文章编号： | 1672-8246(2005)01-0057-03 |
| 修稿时间： | 2004年10月24 |
Research on Rapid Determination Method of Diosgenin in Dioscorea zingiberensis |
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| YI Zi-li,DONG Jing-zhou,JIANG Jian-xiong.Research on Rapid Determination Method of Diosgenin in Dioscorea zingiberensis[J].Journal of West China Forestry Science,2005,34(1):57-59. |
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| Authors: | YI Zi-li DONG Jing-zhou JIANG Jian-xiong |
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| Abstract: | Based on the traditional determination method of Diosgenin , a new rapid determination method of Diosgenin in Dioscorea zingiberensis, microscale extration spectrophotography, was established through research. The traditional method of return flow extraction was replaced by the new method in which the micro sample was lixiviated in anhydrous alcohol. In this new method, the composition of the color-developing agent is anisaldehyde and vitriol, and the solvent is glacial acetic acid. The Absorption of the sample was measured at the wavelength of 460 nm by a spectrophotometer, then the Diosgenin content of the sample was determined according to the standard curve. Compared with the traditional determination method of Diosgenin such as weight method and extraction spectrophotography method , the new method is simple, accurate, low-cost and high-efficient. |
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| Keywords: | Dioscorea zingiberensis Diosgenin rapid determination |
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