Cross-transferable polymorphic SSR loci in Prunus species |
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| Authors: | A Wünsch |
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| Institution: | Unidad de Fruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Ctra. Montañana 930, 50059 Zaragoza, Spain |
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| Abstract: | This work reports the transferability and polymorphism of previously reported SSRs in 10 Prunus species. The availability of a large number of SSRs in the genus Prunus makes marker choice random, while preventing comparison of results in fingerprinting studies. The availability of SSR markers, polymorphic in a wide sample of Prunus species, would facilitate marker choice, while allowing the comparison of results. In this work, microsatellite markers useful for analyzing 10 different Prunus species (P. persica, P. dulcis, P. armeniaca, P. domestica, P. insititia, P. salicina, P. cerasifera, P. avium, P. cersus and P. mahaleb) were searched through screening SSRs previously reported to be conserved and/or polymorphic in more than one Prunus species. A selected group of 13 SSRs, transferable to the 10 species, was analyzed in terms of their usefulness for analyzing these species. The amplification range, polymorphism and variability detected by these loci are reported. The information provided will be useful for Prunus genetic studies as well as conservation and management of Prunus germplasm resources. |
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| Keywords: | Microsatellites Cultivar identification Transferability Prunus persica Prunus dulcis Prunus armeniaca Prunus domestica Prunus insititia Prunus salicina Prunus cerasifera Prunus avium Prunus cersus Prunus mahaleb |
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