| 北运蔬菜基地辣椒病毒病病原种类的分子
检测 |
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| 引用本文： | 姚玉荣,陈国华,冯兰香,谢丙炎.北运蔬菜基地辣椒病毒病病原种类的分子
检测[J].中国蔬菜,2013,1(10):84-89. |
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| 作者姓名： | 姚玉荣 陈国华 冯兰香 谢丙炎 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，农业部蔬菜遗传改良重点开放实验室，北京100081 |
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| 基金项目： | 现代农业产业技术体系建设专项资金资助项目（CARS-25） |
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| 摘 要： | 辣椒病毒病是广东、海南两省北运蔬菜基地的主要病害，十分有必要对其病原 种类进行全面
检测，以便为病毒 病的防治提供依据。本试验运用RT-PCR 方法对这两省北运蔬菜基地的辣椒病毒病或疑
似病毒病的病株样本进行分子检测。结果表明：广东省的病毒检出株率为55.67%，最主要的病毒种类是
黄瓜花叶病毒（CMV）和辣椒轻斑驳病毒（PMMoV），另有少量的马铃薯Y 病毒（PVY）、马铃薯 X 病毒（PVX）
和番茄黄化曲叶病毒（TYLCV）；海南省的病毒检出株率为44.67%，最主要的病毒种类仍然是CMV 和
PMMoV，另有少量的PVY。在田间，两省均以CMV 和PMMoV 复合侵染辣椒为主。
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| 关 键 词： | 广东省 海南省 辣椒病毒病 病原种类 RT-PCR |
Molecular Detection of Pepper Viruses in Southern Vegetable Production
Bases |
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| YAO Yu-Rong,CHEN Guo-Hua,FENG Lan-Xiang,XIE Bing-Yan.Molecular Detection of Pepper Viruses in Southern Vegetable Production
Bases[J].China Vegetables,2013,1(10):84-89. |
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| Authors: | YAO Yu-Rong CHEN Guo-Hua FENG Lan-Xiang XIE Bing-Yan |
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| Institution: | Key Laboratory of Horticultural Crops Genetic Improvement， Ministry of Agriculture， Institute of Vegetables and;
Flowers， Chinese Academy of Agricultural Sciences， Beijing 100081， China |
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| Abstract: | Pepper virus disease is a main disease in the southern vegetable production base in
Guangdong and Hainan Provinces．It is necessary to detect the kinds of pathogeny，so as to provide
references for the prevention of virus diseases. In this study，we conducted molecular detection with
RT-PCR method on the samples of pepper virus diseases or suspected virus diseases from these 2
provinces．The results showed that the detection rate of viruses was 55.67% in Guangdong Province. The
main viruses were CMV（ Cucumber mosaic virus） and PMMoV（ Pepper mild mottle virus）．Besides，
there were a few PVY（ Potato virus Y），PVX（ Potato virus X），and TYLCV（ Tomato yellow leaf curl
virus） detected．In Hainan Province，virus detection rate was 44.67%．CMV and PMMoV were the
major viruses．There was also a small quantity of PVY. In the fields，the complex infection of CMV and
PMMoV was the crucial problem in pepper production in Guangdong and Hainan Provinces. |
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| Keywords: | Hainan Province Pepper virus diseases Kinds of pathogeny RT-PCR |
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