| 不同产地毛头鬼伞子实体多糖特征及其刺激巨噬细胞产生NO的活性 |
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| 引用本文： | 赵源,刘艳芳,蒋俊,杨焱,唐庆九,周帅,吴迪,贾薇,冯娜,张劲松.不同产地毛头鬼伞子实体多糖特征及其刺激巨噬细胞产生NO的活性[J].食用菌学报,2011,18(3):57-60. |
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| 作者姓名： | 赵源 刘艳芳 蒋俊 杨焱 唐庆九 周帅 吴迪 贾薇 冯娜 张劲松 |
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| 作者单位： | 1. 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403;南京农业大学生命科学学院,江苏南京210095
2. 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403 |
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| 基金项目： | 上海市科委成果转化项目(编号:113919N1300)的部分研究内容 |
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| 摘 要： | 用水提醇沉法从6个不同产地毛头鬼伞(Coprinus comatus)子实体中获得6种粗多糖,6种粗多糖得率及多糖含量差异较大,以湖北(HB)的最高,其次为云南(YN)的；各粗多糖分子量分布相似,单糖组成均以葡萄糖为主,并含有少量半乳糖；除福建(FJ)外,其余5种粗多糖均能增强小鼠巨噬细胞RAW264.7释放NO的产量,以YN和HN的活性较好,总体来说,YN的多糖产量及活性较好.
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| 关 键 词： | 毛头鬼伞 多糖 NO产量 巨噬细胞 免疫调节 |
Polysaccharide Preparations from Commercially-grown Coprinus comatus Fruit Bodies and Their Effect on Nitric Oxide Release from Macrophages |
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| ZHAO Yuan
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LIU Yanfang
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JIANG Jun
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YANG Yan
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TANG Qingjiu
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ZHOU Shuai
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WU Di
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JIA Wei
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FENG Na
,
ZHANG Jingsong.Polysaccharide Preparations from Commercially-grown Coprinus comatus Fruit Bodies and Their Effect on Nitric Oxide Release from Macrophages[J].Acta Edulis Fungi,2011,18(3):57-60. |
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| Authors: | ZHAO Yuan LIU Yanfang JIANG Jun YANG Yan TANG Qingjiu ZHOU Shuai WU Di JIA Wei FENG Na ZHANG Jingsong |
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| Institution: | ZHAO Yuan1,2,LIU Yanfang1,JIANG Jun1,YANG Yan1,TANG Qingjiu1,ZHOU Shuai1,WU Di1,JIA Wei1,FENG Na1,ZHANG Jingsong1 1National Engineering Research Center of Edible Fungi,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China,2College of Life Sciences,Nanjing Agriculture University,Nanjing,Jiangsu 210095 |
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| Abstract: | Crude polysaccharide preparations were obtained from artificially-cultivated Coprinus comatus fruit bodies,provided by six commercial growers from different locations within China,by hot(100 ℃) water extraction and ethanol precipitation.Percentage yields of crude extracted material from the six samples were HB 9.42,YN 8.89,AH 8.52,HN 7.03,FJ 6.68 and ZJ 5.26,and the corresponding percentage yields of polysaccharide were 7.49,6.68,6.36,5.33,4.35 and 2.84.HPLC analysis revealed that crude polysaccharide material extracted from the six fruit body samples had similar molecular weight distributions,and all contained high amounts of glucose and smaller quantities of galactose.All crude extract samples except for FJ stimulated nitric oxide production by RAW 264.7 macrophages,which was adopted as a measure of immunomodulatory activity,with highest NO released in response to the extracts designated YN and HN.Overall,YN fruit bodies generated relatively high yields of crude extract and polysaccharide,and provided the strongest immunomodulatory response. |
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| Keywords: | Coprinus comatus polysaccharide nitric oxide production macrophages immunoregulation |
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