| 葡萄花丝培养中的胚胎发生 |
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| 引用本文： | 卢炳芝,于向荣,郭飞跃,李佩芬,鲍雪珍.葡萄花丝培养中的胚胎发生[J].果树学报,1995(Z1). |
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| 作者姓名： | 卢炳芝 于向荣 郭飞跃 李佩芬 鲍雪珍 |
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| 作者单位： | 山东省酿酒葡萄科学研究所!济南250100(卢炳芝，于向荣，郭飞跃，李佩芬)，山东大学生物系(鲍雪珍) |
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| 摘 要： | 葡萄花丝在含6—BA2mg·1~(-1)+2,4—Dlmg·1~(-1)的B_5培养基上,培养20d发生绿色、结构紧密、坚硬凸起不规则胚性愈伤组织,诱导率达30％;将此愈伤组织转入含6—BA0.5mg·1~(-1)的B_5培养基上,30d后分化出胚状体;将胚状体转入含6—BA0.3mg·1~(-1)+NAA0.01mg·1~(-1)的B_5培养基上,30d后萌发成苗。
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| 关 键 词： | 葡萄花丝 胚性愈伤组织 胚状体 花丝幼苗 |
The Embryogenesis from Culture in vitro of Filaments of Grapevine |
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| Lu Bingzhi,Yu Xiangrong,Guo Feiyue,and Li Peifen.The Embryogenesis from Culture in vitro of Filaments of Grapevine[J].Journal of Fruit Science,1995(Z1). |
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| Authors: | Lu Bingzhi Yu Xiangrong Guo Feiyue and Li Peifen |
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| Abstract: | The green, tight embryogenic calli from the filaments of grapevine (Vitis vinifera L. cv Muscat Hamburg etc)formed on B5 medium with 1 mg ·1-12,4-D,2 mg·1-16BA,after the filaments were cultured 20 days. The embryogenic calli induced rate is 30% . When the embryogenic calli were transferred on B5 medium with 0. 5mg·1-16BA,the embryoids were produced after 30 days. Then plantlet regeneration from the embryoids formed about 30 days later on B5 medium with 0. 3 mg·1-16BA,0. 01 mg·1-1NAA. |
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| Keywords: | Filament Embryogenic calli Embryoid Plantlet |
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