| 黄芩过氧化物酶同工酶几种酶液提取方法的比较 |
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| 引用本文： | 张东向,张磊,王蕊.黄芩过氧化物酶同工酶几种酶液提取方法的比较[J].北方园艺,2008(6). |
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| 作者姓名： | 张东向 张磊 王蕊 |
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| 作者单位： | 齐齐哈尔大学,生命科学与工程学院,黑龙江,齐齐哈尔161006 |
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| 基金项目： | 黑龙江省高校骨干教师创新能力资助计划 |
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| 摘 要： | 利用聚丙烯酰胺凝胶电泳对黄芩(Scutellaria baicalensis Georgi)萌发的种子,愈伤组织,悬浮细胞及幼苗过氧化物酶几种酶液提取方法进行比较.结果表明:各种酶液提取方法对不同部位的同工酶提取效果不同.同工酶是研究植物多样性的有力工具,过氧化物酶则是广泛存在于植物体内的一种重要的同工酶,能反映植物生长发育特点、体内代谢状况及对外界环境的适应.
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| 关 键 词： | 黄芩 过氧化物同工酶 电泳 |
Comparison On Several Extraction Methods of Enzyme Solution of Peroxidase and Isoenzyme of Scutellaria baicalensis Ceorm |
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| ZHANG Dong-xiang,ZHANG Lei,WANG Rui.Comparison On Several Extraction Methods of Enzyme Solution of Peroxidase and Isoenzyme of Scutellaria baicalensis Ceorm[J].Northern Horticulture,2008(6). |
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| Authors: | ZHANG Dong-xiang ZHANG Lei WANG Rui |
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| Abstract: | In this paper,some methods of extracting peroxidase isoenzymes in germinating seeds, callus tissue, suspension cells and seedlings of Scutellaria baicalensis Georgi were analyzed through electrophoresis.The results indicated that every method of extracting peroxidase isoenzymes in different sites has different effect. Isoenzyme was a effective tool in reseaching plant variety. Peroxidase isoenzyme was a important isoenzyme distributing widely in the plant, could responds the characters of plant growing and development, the internal metabolism situation and the adaptation to external envirement. |
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| Keywords: | Scutellaria baicalensis Georgi Peroxidase isoenzyme Electrophoresis |
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