Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations

Authors:	Antoine LF Gady Freddy WK Hermans Marion HBJ Van de Wal Eibertus N van Loo Richard GF Visser Christian WB Bachem

Institution:	(1) Wageningen UR, Plant Breeding, Wageningen University and Research Center, PO box 386, 6700, AJ, Wageningen, The Netherlands;(2) Graduate School Experimental Plant Sciences, Building RADIX - West (building nr 107), Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands;(3) Nunhems Netherlands BV, PO Box 4005, 6080, AA, Haelen, The Netherlands

Abstract:	Background The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as a model for berry-fruit plants. With the progress of the tomato sequencing project, reverse genetics becomes an obvious and achievable goal.

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