Bone marrow mesenchymal stem cells are induced to differentiate into cardiomyocytes by hepatocyte growth factor in vitro |
| |
| Authors: | FANG Tian-ling MIN Jun DENG Xiao-geng QIAN Shi-kun CHU Zhong-hua CHEN Ya-jin SHAO Jing WEI Jing CHEN Ji-sheng |
| |
| Institution: | 1. Department of Hepatobilary Surgery, Second Affiliated Hospited, Sun Yat-sen University, Guangzhou 510120, China;
2. Stem Cell Research Center, Research Center of Medicine, Second Affiliated Hospited, Sun Yat-sen University, Guangzhou 510120, China |
| |
| Abstract: | AIM: To explore a new method of hepatocyte growth factor (HGF) inducing bone marrow mesenchymal stem cells (MSC) to differentiate into cardiomyocytes. METHODS: Bone marrow MSC was cultured with DMEM media (10% fetal calf serum) 4-6 passages, and induced by HGF (10 μg/L) for 30 d. Automatical beating of the differentiated cells was observed daily with transverse microscopy, or under condition of 0.1% isoproterenol or cal-cium-deprived incubation. Specific cardiac myosin in the cells was indentified by immunochemistry. RESULTS: At 14-20 d of differentiation, bone marrow mesenchymal stem cells formed clones, in 10%-50% of which spontaneous beating cell-mass had come to continuously exist. Isoproterenol increased the beating rate and calcium-deprived media inhibited the beating. The cells were identified to be cardiomyocytes by expression of cardiac myosin heavy chain. CONCLUSION: HGF may induce bone marrow mesenchymal stem cells into cardiomyocytes with high efficiency, but the differentiating pathway of stem cells remains to be further studied. |
| |
| Keywords: | Hepatocyte growth factor Bone marrow mesenchymal stem cells Myocardium Differentiation |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
