| 西瓜抗病毒基因zym-CH的比较图谱定位 |
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| 引用本文： | 徐向丽,郭绍贵,宫国义,许勇,卢向阳,彭振兴,张海英.西瓜抗病毒基因zym-CH的比较图谱定位[J].园艺学报,2009,36(2):227-232. |
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| 作者姓名： | 徐向丽 郭绍贵 宫国义 许勇 卢向阳 彭振兴 张海英 |
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| 作者单位： | （ 湖南农业大学生物科技学院，长沙410128；国家蔬菜工程技术研究中心，北京100097） |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 以西瓜抗ZYMV-CH（小西葫芦黄花叶病毒中国株系，Zucchini Yellow Mosaic Virus-CHINA）野生种质P.I.595203和感病自交系98R为试材构建F2群体和F2:3群体，采用BSA法筛选与抗病基因zym-CH连锁的分子标记，并利用以F2S8群体（P.I.296341-FR×97103）构建的西瓜参考遗传图谱进行zym-CH的比较定位。试验结果表明：在参考遗传图谱已经定位的RAPD和SSR标记中，RAPD标记b13-1400与zym-CH的遗传距离为25.6 cM；SSR标记MCPI-14和 MCPI-26与zym-CH的遗传距离分别为57 cM和87 cM，比较分析发现这些标记在两个图谱上的分布呈共线性关系。根据zym-CH与标记b13-1400、MCPI-14和MCPI-26之间的遗传关系，将西瓜抗病毒病基因zym-CH初步定位在西瓜参考遗传图谱3号连锁群105 cM处。
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| 关 键 词： | 西瓜 zym-CH BSA法 共线性 比较定位 |
| 收稿时间： | 2008-7-20 |
| 修稿时间： | 2008-11-17 |
Comparative Mapping of Virus Resistant Gene zym-CH in Watermelon |
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| XU Xiang-li,GUO Shao-gui,GONG Guo-yi,XU Yong,LU Xiang-yang,PENG Zhen-xing,ZHANG Hai-ying.Comparative Mapping of Virus Resistant Gene zym-CH in Watermelon[J].Acta Horticulturae Sinica,2009,36(2):227-232. |
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| Authors: | XU Xiang-li GUO Shao-gui GONG Guo-yi XU Yong LU Xiang-yang PENG Zhen-xing ZHANG Hai-ying |
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| Institution: | (College of Bioscience and Biotechnology, Hunan Agriculture University,Changsha 410128, China; National Engineering Research Center for Vegetables, Beijing 100097, China) |
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| Abstract: | The watermelon germplasm P.I.595203 resistant to ZYMV-CH ( Zucchini Yellow Mosaic Virus-CHINA ) and 98R susceptible to ZYMV-CH were used as the parents to construct F2 population and F2:3 population. The markers linked to the resistant gene zym-CH were filtered by BSA strategy.The reference genetic map constructed basing on the F2S8 population（P.I.296341-FR×97103）was used for comparative mapping of zym-CH gene. The results showed that b13-1400, MCPI-14 and MCPI-26 were linked to zym-CH gene with the genetic distance of 25.6 cM, 57cM and 87cM respectively. The resistant gene zym-CH was located on 105 cM of the linkage group 3 by the collinearity relationship of zym-CH, b13-1400, MCPI-14 and MCPI-26 in the two linkage maps. |
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| Keywords: | zym-CH |
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