| 番茄匍柄霉叶斑病拮抗细菌的筛选与鉴定 |
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| 引用本文： | 李新宇,李 磊,陈利达,石延霞,柴阿丽,谢学文,李宝聚.番茄匍柄霉叶斑病拮抗细菌的筛选与鉴定[J].园艺学报,2020,36(4):741-748. |
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| 作者姓名： | 李新宇 李 磊 陈利达 石延霞 柴阿丽 谢学文 李宝聚 |
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| 作者单位： | 1中国农业科学院蔬菜花卉研究所，北京 100081；2沈阳农业大学植物保护学院，沈阳 110866 |
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| 基金项目： | 国家重点研发计划项目（2017YFD0201102）；中国农业科学院科技创新工程项目（CAAS-ASTIP-IVFCAAS）；农业部园艺作物生物学与种质创制重点实验室项目 |
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| 摘 要： | 应用平板对峙法从连作多年的番茄根际土样中筛选得到对番茄匍柄霉叶斑病具有较强拮抗活性的细菌菌株ZF161，该菌株对番茄匍柄霉的平板抑制率达到70.51%。离体叶片试验显示，菌株ZF161对番茄匍柄霉叶斑病防治效果达到69.83%。通过菌落形态观察、生理生化特性、Biolog测定和多基因系统发育树综合分析，鉴定菌株ZF161为枯草芽孢杆菌（Bacillus subtilis）。番茄盆栽试验结果表明，菌株ZF161对番茄匍柄霉叶斑病的防治效果达到63.27%。进一步平板对峙抑菌谱试验结果显示，菌株ZF161对其他7种病原真菌也具有较好的抑制效果。上述结果说明，菌株ZF161对番茄匍柄霉叶斑病具有良好的防治效果，且对多种病原真菌也具有抑制作用，具有开发应用的潜力。
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| 关 键 词： | 番茄 拮抗细菌 枯草芽孢杆菌 番茄匍柄霉叶斑病 防治效果 |
Progress in DNA double-strand break repair regulated by PIKK |
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| YAO De-shan,ZHANG Zhen-gang,GONG Kai-zheng.Progress in DNA double-strand break repair regulated by PIKK[J].Acta Horticulturae Sinica,2020,36(4):741-748. |
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| Authors: | YAO De-shan ZHANG Zhen-gang GONG Kai-zheng |
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| Institution: | 1.Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Plant Protection College，Shenyang Agricultural University，Shenyang 110866，China |
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| Abstract: | DNA damage presents a major threat to genome stability. Repairing damaged DNA is essential for maintaining genome integrity and preventing genome instability-related diseases, such as cancer. DNA damage response is a multicomplex network of signaling pathways involved in DNA damage repair, cell cycle checkpoints and apoptosis, which mediates the cellular response to endogenous and exogenous stresses. DNA double-strand break is the most serious form of DNA damage. Three members of the phosphatidylinositol 3-kinase (PI3K)-related kinase (PIKK) protein family, DNA-PK, ATM and ATR, are key effectors of DNA damage response and are extensively linked to tumourigenesis and survival of cancer cells after radio-/chemotherapy. This article mainly discusses their functions, and mechanisms of activation and regulation. |
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| Keywords: | DNA damage response DNA double-strand breaks DNA-dependent protein kinase ATM protein ATR protein |
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