| 花烛体细胞胚胎发生及植株再生研究 |
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| 引用本文： | 辛伟杰,徐彬,王广东,郭维明,文方德,金剑平.花烛体细胞胚胎发生及植株再生研究[J].园艺学报,2006,33(6):1281-1286. |
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| 作者姓名： | 辛伟杰 徐彬 王广东 郭维明 文方德 金剑平 |
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| 作者单位： | (南京农业大学园艺学院, 江苏南京210095; 珠海市园艺研究所, 广东珠海519070) |
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| 基金项目： | 国家自然科学基金项目(30300244) |
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| 摘 要： | 以花烛(Anthurium andraeanum Lind. ) 盆花品种无菌苗叶片、叶柄、根段为外植体, 对花烛体细胞胚胎发生及植株再生进行了研究, 建立了花烛体细胞胚胎发生体系。结果表明, 花烛叶片较其他外植体更适宜体细胞胚的诱导; 胚性愈伤组织及胚性结构诱导最适培养基为改良MS + 2, 4-D 2.0～3.0 mg·L - 1 + KT 0.5 mg·L - 1 +蔗糖2% +葡萄糖1% , pH 5.5, 暗培养40 d, 诱导率可达90.3%; 体胚发育适宜培养基为改良MS + 6-BA 0.5 mg·L - 1 +蔗糖3% , 光强20 μmol·m -2 · s-1 , 培养20 d后体胚萌发率达6219%; 萌发的体胚在发育培养基上继续培养30 d后均能发育成完整植株。对不同阶段培养材料的组织结构及超微结构的观察证明了花烛体胚发生过程。
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| 关 键 词： | 花烛 体细胞胚 诱导 发育 植株再生 |
| 文章编号： | 0513-353X（2006）06-1281-06 |
| 收稿时间： | 2006-01-16 |
| 修稿时间： | 2006-01-162006-04-11 |
Somatic Embryogenesis and Plant Regeneration of Anthurium andraeanum |
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| Xin Weijie,Xu Bin,Wang Guangdong,Guo Weiming,Wen Fangde,Jin Jianping.Somatic Embryogenesis and Plant Regeneration of Anthurium andraeanum[J].Acta Horticulturae Sinica,2006,33(6):1281-1286. |
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| Authors: | Xin Weijie Xu Bin Wang Guangdong Guo Weiming Wen Fangde Jin Jianping |
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| Institution: | (College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; Zhuhai Institute of Horticulture,Zhuhai, Guangdong 519070, China) |
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| Abstract: | A protocol for somatic embryogenesis and plant regeneration of Anthurium andraeanum Lind. in vitro plants has been successfully developed. Leaf sections, petioles and root sections were used as explants, the results showed that leaf sectionswere suitable exp lants and expressed higher embryogenic potential than others; Up to 9013% of explants produced embryogenic calli and embryogenic structures after 40 days culture on modified MS medium supplemented with 2.0 to 3.0 mg·L -1 2, 4-D, 0.5 mg·L -1 KT, 2% sucrose and 1% glucose; 62.9% of somatic embryos developed after 20 days culture on modified MS medium containing 0.5 mg·L - 1 6-BA and 3% sucrose under light conditions of 20 μmol·m- 2 ·s-1 and converted into plantlets with shoots and roots after 30 days culture on the same medium. The observation of histological structure and ultrastructure proved the process of somatic embryogenesis of Anthurium. |
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| Keywords: | Anthurium andraeanum Somatic embryo Induction Development Plant regeneration |
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