| 农杆菌介导的蝴蝶兰基因转化系统的建立 |
| |
| 引用本文： | 柴明良,金斗焕.农杆菌介导的蝴蝶兰基因转化系统的建立[J].园艺学报,2004,31(4):537-540. |
| |
| 作者姓名： | 柴明良 金斗焕 |
| |
| 作者单位： | ( 浙江大学园艺系，杭州310029； 韩国建国大学园艺系，汉城143—701) |
| |
| 摘 要： | 针刺后的蝴蝶兰‘White Hikaru’的类原球茎(PLB)，与含绿色荧光蛋白基因( )和潮霉素磷酸转移酶基因(^p￡)的pCAMBIA 1300一SmGFP的根瘤农杆菌LBA4404 共培养，培育出了转基因的蝴蝶兰。经绿色荧光蛋白检测和Southern印迹，证实了再生植株中含cop基因和hpt基因。
|
| 关 键 词： | 蝴蝶兰 类原球茎 农杆菌介导的转化 绿色荧光蛋白基因 潮霉素磷酸转移酶基因 |
| 文章编号： | 0513-353X(2004)04-0537-03 |
Establishment of Agrobacterium-mediated Transformation in Phalaenopsis |
| |
| Chai Mingliang and Kim Doohwan.Establishment of Agrobacterium-mediated Transformation in Phalaenopsis[J].Acta Horticulturae Sinica,2004,31(4):537-540. |
| |
| Authors: | Chai Mingliang and Kim Doohwan |
| |
| Institution: | Institute of Hygiene & Environmental Medicine, Academy of Military Medical Science, Tianjin 300050, China |
| |
| Abstract: | Transgenic orchid (Phalaenopsis cv. White Hikaru) plants were regenerated by inoculating a needle wounded protocorm-like body ( PLB) with Agrobacterium tumefaciens strain LBA4404 harboring pCAMBIA 1300-SmGFP that carries green fluorescent protein gene (gfp) and hygromycin phosphotransferase gene ( hpt) . The expression and presence of the transgenes, gfp and hpt were assessed by detection of green fluorescent protein under microscope and Southern analysis, respectively. |
| |
| Keywords: | Phalaenopsis Protocorm-like body (PLB) Agrobacterium-medialed transformation Green fluorescent protein gene (gfp) Hygromycin phosphotransferase gene (hpt) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
