Quercetin regulates Fas expression and induces apoptosis in hepatocellular carcinoma HepG2 cells |
| |
| Authors: | ZHANG Ji-hong LIANG Li-jian HUANG Jie-fu |
| |
| Institution: | 1.Division of Hepato-Biliary Surgery, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China;2.Department of Surgery, Clinical College of Guangdong Pharmaceutical University, Guangzhou 510224, China;3.Division of Hepato-Biliary Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China. E-mail: zhjihong@hotmail.com |
| |
| Abstract: | AIM: To investigate the role of 1, 4, 5- trisphosphate inositol (IP3) and Fas gene expression in apoptosis of HepG2 cells induced by quercetin. METHODS: HepG2 cells were treated with quercetin at different concentrations (including 20, 40, 60, 80 μmol/ L) for 72 h and treated with 60 μmol/ L quercetin for 6 h, 12 h, 24 h, 48 h and 72 h. IP3, Fas mRNA, Fas protein and apoptosis rate were assayed by IP3 - ［3H］ Birtrak assay, RT-PCR, Western blotting and flow cytometry, respectively. RESULTS: When HepG2 cells were incubated with different concentrations of quercetin for 72 h, the IP3 content was lower than those in control. Fas mRNA expression, Fas protein expression and the apoptosis rate were higher than those in control. When HepG2 cells were incubated with quercetin for 6 h, 12 h, 24 h, 48 h, 72 h, the IP3 contents were lower than those in control incubated with 60 μmol/L quercetin for 12 h. Fas mRNA expression was higher than that in control incubated with 60 μmol/L quercetin for 12 h . Fas protein expression was higher than that in control. The apoptosis rate was significantly higher than that in control incubated with 60 μmol/L quercetin for 24 h (P<0.01). CONCLUSION: Quercetin induces apoptosis of HepG2 cells by reducing IP3 production and upregulating Fas gene expression. |
| |
| Keywords: | Carcinoma hepatocellular Quercetin Genes Fas Apoptosis Inositol 1 4 5-triphosphate |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
