| 洋葱S型细胞质雄性不育相关基因的挖掘及分子标记开发 |
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| 引用本文： | 陈立,杨亚会,霍雨猛,刘冰江,孙雨晴,杨建平,吴雄,杨妍妍.洋葱S型细胞质雄性不育相关基因的挖掘及分子标记开发[J].园艺学报,2018,45(3):503-510. |
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| 作者姓名： | 陈立 杨亚会 霍雨猛 刘冰江 孙雨晴 杨建平 吴雄 杨妍妍 |
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| 作者单位： | （1山东农业大学园艺科学与工程学院，山东泰安 271018；2山东省农业科学院蔬菜花卉研究所，济南 250100；3吉林农业大学园艺学院，长春 130118） |
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| 摘 要： | 以洋葱（Allium cepa L.）S型细胞质雄性不育系和保持系材料为试材，对洋葱线粒体全基因组中的58个开放阅读框（ORF）进行差异表达分析，发现了一个表达差异片段orf393。采用实时荧光定量PCR技术，利用洋葱S型细胞质雄性不育系和保持系的根，叶片，花茎以及花粉母细胞、四分体、单核花粉粒、成熟花粉粒等4个发育时期的花蕾对orf393进行时空表达分析。结果表明：orf393在洋葱S型细胞质雄性不育系的根、叶片、花茎和4个发育时期的花蕾中均有表达，尤其是在四分体时期花蕾中表达量最高，之后恢复到营养生长时期的表达水平，但在保持系中未见其表达，这表明orf393是组成型表达，可能与洋葱的花粉发育存在某种关联。利用orf393在S型细胞质雄性不育系和保持系之间的序列差异，开发了一个稳定可靠的鉴别洋葱细胞质类型的SCAR分子标记GXX393。
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| 关 键 词： | 洋葱 细胞质雄性不育 orf393 基因表达 分子标记 |
Gene Discovery Related to S-type Male Sterility in Onion and Its Usage for Development of Molecular Marker |
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| CHEN Li,YANG Yahui,HUO Yumeng,LIU Bingjiang,SUN Yuqing,YANG Jianping,WU Xiong,YANG Yanyan,.Gene Discovery Related to S-type Male Sterility in Onion and Its Usage for Development of Molecular Marker[J].Acta Horticulturae Sinica,2018,45(3):503-510. |
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| Authors: | CHEN Li YANG Yahui HUO Yumeng LIU Bingjiang SUN Yuqing YANG Jianping WU Xiong YANG Yanyan |
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| Institution: | 2.Institute of Vegetables and Flowers，Shandong Academy of Agricultural Sciences，Ji’nan 250100，China；3College of Horticulture，Jilin Agricultural University，Changchun 130118，China） |
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| Abstract: | The differential expression of 58 open reading frames（ORFs）identified in the intergenic sequence of the mitochondrial genome was analyzed using a male sterile line and a maintainer line of onion（Allium cepa L.），through which，a differentially expressed fragment was obtained，designated as orf393. By quantitative real-time PCR，the temporal-spatial expression of orf393 was carried out in different tissues and organs，including roots，leaves，stalks，and the buds of different developmental stages （pollen mother cells，tetrad，mononuclear，and matured pollen grains）. The results showed that orf393 was only expressed in the samples of the male sterile line；its expression could be detected in all samples of the male sterile line，especially the buds during the tetrad stage，which had the highest expression of orf393. The expression level of orf393 returned to the same level as that during the vegetative stage. Our results suggest that orf393 is constitutively expressed and may associate with the male sterility of onion. Based on the sequence difference of orf393 between the male sterile line and its maintainer line，we developed a reliable molecular marker，namely GXX393，for the identification of cytoplasm types of onion. |
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| Keywords: | onion cytoplasmic male-sterility orf393 gene expression molecular marker |
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