| 枯草芽孢杆菌SNB-86菌肥对连作平邑甜茶幼苗生长及土壤环境的影响 |
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| 引用本文： | 刘丽英,刘珂欣,迟晓丽,张潇,许超,朱浩,金晓,刘维维,孙中涛,毛志泉.枯草芽孢杆菌SNB-86菌肥对连作平邑甜茶幼苗生长及土壤环境的影响[J].园艺学报,2018,45(10):2008-2018. |
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| 作者姓名： | 刘丽英 刘珂欣 迟晓丽 张潇 许超 朱浩 金晓 刘维维 孙中涛 毛志泉 |
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| 作者单位： | 1山东农业大学生命科学学院，山东泰安 271018；2山东农业大学园艺科学与工程学院/作物生物学国家重点实验室，山东泰安 271018 |
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| 基金项目： | 国家现代农业产业技术体系建设专项资金项目（CARS-28）；山东省重点研发计划项目（2017CXGC0306）；山东省“双一流”建设项目（SYL2017XTTD15） |
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| 摘 要： | 采用对峙法从苹果连作土壤中分离得到一株连作生防细菌SNB-86,鉴定为枯草芽孢杆菌(Bacillus subtilis)。对该菌进行固态发酵制备菌肥。以苹果连作土盆栽平邑甜茶幼苗为材料,对盆栽土壤设置4个处理:不做任何处理(对照)、连作土经溴甲烷熏蒸、连作土中加入菌肥载体处理和枯草芽孢杆菌SNB-86菌肥处理。试验结果表明:SNB-86菌肥处理下连作平邑甜茶幼苗鲜质量和干质量分别增加了171.5%和142.3%,株高分别比对照和菌肥载体处理增加了97.9%和25.3%,地径增加了57.6%和6.2%,但SNB-86菌肥处理的幼苗长势均未超过溴甲烷处理。菌肥载体处理和SNB-86菌肥处理土壤中细菌和放线菌的数量都增加,SNB-86菌肥处理土壤真菌的数量显著降低,为对照中的32.3%,而菌肥载体处理仅比对照组降低了10%。溴甲烷熏蒸处理显著降低了土壤中微生物的数量,导致土壤酶活性低于对照。SNB-86菌肥处理和菌肥载体处理可显著提高连作土壤中蔗糖酶、脲酶、过氧化氢酶、中性磷酸酶的活性,菌肥载体处理次之;而溴甲烷熏蒸处理降低了这些酶的活性。SNB-86菌肥可以显著促进连作条件下平邑甜茶幼苗的生长,提高连作土壤酶活性,降低土壤中可培养真菌的数量,在一定程度上能缓解苹果连作障碍。
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| 关 键 词： | 平邑甜茶 连作障碍 SNB-86菌肥 土壤酶活性 |
Effects of Bioorganic Fertilizer SNB-86 Special for Continuous Cropping Apple on Malus hupehensis Seedlings and Soil Environment Under Replant Disease Condition |
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| LIU Liying,LIU Kexin,CHI Xiaoli,ZHANG Xiao,XU Chao,ZHU Hao,JIN Xiao,LIU Weiwei,SUN Zhongtao,MAO Zhiquan,.Effects of Bioorganic Fertilizer SNB-86 Special for Continuous Cropping Apple on Malus hupehensis Seedlings and Soil Environment Under Replant Disease Condition[J].Acta Horticulturae Sinica,2018,45(10):2008-2018. |
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| Authors: | LIU Liying LIU Kexin CHI Xiaoli ZHANG Xiao XU Chao ZHU Hao JIN Xiao LIU Weiwei SUN Zhongtao MAO Zhiquan |
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| Institution: | 1College of Life Science，Shandong Agricultural University，Tai'an，Shandong 271018，China；2State Key Laboratory of Crop Biology，College of Horticulture Science and Engineering，Shandong Agricultural University，Tai'an，Shandong 271018，China |
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| Abstract: | A biocontrol bacteria SNB-86 isolated from apple continuous cropping soil by confrontation method was identified as Bacillus subtilis. The biofertilizer was prepared by solid-state fermentation using SNB-86，and pot experiment was carried out with apple continuous soil potted Malus hupehensis Rehd. seedlings as the material. Four treatments were set：continuous cropping soil（control），continuous cropping soil fumigated with methyl bromide，biofertilizer carrier and SNB-86 biofertilizer treated soil in pot. The results showed that bioorganic fertilizer SNB-86 significantly increased 171.5% fresh weight and 142.3% dry weight of M. hupehensis seedlings，respectively. The M. hupehensis seedling height increased 97.9% and 25.3% in SNB-86 biofertilizer treated group than that in continuous cropping soil and biofertilizer carrier groups，respectively. The M. hupehensis seedling basal diameter increased 57.6% and 6.2% in SNB-86 biofertilizer treated group than that in continuous cropping soil and biofertilizer carrier groups，respectively. The growth of M. hupehensis seedling in SNB-86 biofertilizer treated group was not good as that in continuous cropping soil fumigated with methyl bromide group. Compared with continuous cropping soil，the number of soil bacteria and actinomycetes in biofertilizer carrier and SNB-86 biofertilizer treated groups increased. The number of soil fungi in SNB-86 biofertilizer treated group decreased significantly to 32.3% of continuous cropping soil，while biofertilizer carrier treatment had less influence on the number of fungi in continuous cropping soil，only 10% lower than that of the continuous cropping soil group. But bromomethane fumigation significantly reduced the number of microbes in soil，resulting in lower soil enzyme activity than continuous cropping soil group. The activities of sucrase，urease，catalase and neutral phosphatase were significantly increased by SNB-86 biofertilizer treatment and biofertilizer carrier treatment. The effect of SNB-86 biofertilizer treatment was most obvious，followed by biofertilizer carrier treatment. While the activities of sucrase，urease，catalase and neutral phosphatase in continuous cropping soil fumigated with methyl bromide were significantly decreased，respectively compared with that in continuous cropping soil group. It is concluded that SNB-86 biofertilizer can significantly promote the growth of M. hupehensis seedlings，increase the activity of soil enzymes，reduce the number of culturable fungi in the soil，and alleviate the obstacle of apple continuous cropping. |
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| Keywords: | Malus hupehensis replant disease SNB-86 fertilizer soil enzyme activity |
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