| 瓜叶绿素降解关键酶基因PPH和PAO cDNA片段的克隆与表达初步分析 |
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| 引用本文： | 王伟,徐跃进,万正杰.瓜叶绿素降解关键酶基因PPH和PAO cDNA片段的克隆与表达初步分析[J].园艺学报,2011,38(6):1104-1110. |
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| 作者姓名： | 王伟 徐跃进 万正杰 |
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| 作者单位： | （华中农业大学园艺林学学院，国家蔬菜改良中心华中分中心，园艺植物生物学教育部重点实验室，武汉 430070） |
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| 基金项目： | 国家‘948’项目(2008-Z26); 湖北省科技攻关项目(20066109) |
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| 摘 要： | 以西双版纳黄瓜和‘华黄5号’黄瓜叶片为试验材料，研究了衰老过程中两种黄瓜叶片叶绿素含量变化及脱镁叶绿素脱镁叶绿酸水解酶基因PPH和脱镁叶绿酸a加氧酶基因PAO的表达。结果表明：随着叶片衰老，叶绿素a和叶绿素b含量显著降低，叶绿素a与叶绿素b的比值在0.5左右，推测叶绿素b的含量可能对黄瓜叶片保绿起到重要作用。荧光定量RT-PCR分析结果显示：PPH和PAO在西双版纳黄瓜叶片生长35 d时表达量最高，分别为5.50和1.86；PPH在‘华黄5号’叶片35 d时表达量最高，为7.14，而PAO在35和45 d表达量都很高，分别为3.90和3.91；PPH和PAO在西双版纳黄瓜叶片中的表达量低于在‘华黄5号’中。
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| 关 键 词： | 黄瓜 叶绿素 降解 PPH PAO 衰老 表达 |
| 收稿时间： | 2011-01-11 |
Cloning and Expression Analysis of Key Genes PPH and PAO for Chlorophyll Degradation in Cucumber |
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| WANG Wei,XU Yue-jin,WAN Zheng-jie.Cloning and Expression Analysis of Key Genes PPH and PAO for Chlorophyll Degradation in Cucumber[J].Acta Horticulturae Sinica,2011,38(6):1104-1110. |
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| Authors: | WANG Wei XU Yue-jin WAN Zheng-jie |
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| Institution: | （College of Horticulture and Forestry，Huazhong Agricultural University；National Center for Vegetable Improvement（Centeral China）；Key Laboratory of Horticulture Plant Biology，Ministry of Education，Wuhan 430070，China） |
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| Abstract: | The change of chlorophyll content and the expression of pheophytin pheophorbide hydrolase gene(PPH)and pheidea oxygenase gene(PAO)were analyzed during leaf senescence of Cucumis sativus L.var.xishuangbannanesis Qi et Yuan and'Huahuang 5'.The results showed that chlorophyll a and b content decreased significantly during the leaf senescence.The ratio of chlorophyll a and b was around 0.5,suggesting that chlorophyll b content might play a role in keeping green.Real-time PCR results showed that PPH and PAO had ... |
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| Keywords: | cucumber chlorophyll breakdown PPH PAO senescence expression |
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