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结球甘蓝抽薹性遗传规律和QTL定位分析
引用本文:王五宏,汪精磊,李必元,魏庆镇,胡天华,胡海娇,包崇来.结球甘蓝抽薹性遗传规律和QTL定位分析[J].园艺学报,2020,47(5):974-982.
作者姓名:王五宏  汪精磊  李必元  魏庆镇  胡天华  胡海娇  包崇来
作者单位:浙江省农业科学院蔬菜研究所,杭州 310021
基金项目:国家自然科学基金项目(31372058);浙江省重大科技专项(2012C12903-6)
摘    要:以早抽薹‘S-1’与晚抽薹‘G-1’为亲本,构建F1、BC1、BC2和F2群体,采用植物数量性状主基因 + 多基因混合遗传模型分析法对结球甘蓝抽薹性状进行遗传分析,并采用SLAF-BSA方法对抽薹时间进行QTL定位分析。结果显示,结球甘蓝抽薹性状是由2对加性—显性—上位性主基因 + 加性—显性多基因遗传控制;主基因 + 多基因平均遗传率是93.41%。共检测到2个QTL,分别为2号染色体上的2.31 ~ 3.09 Mb和33.57 ~ 34.40 Mb,总长度为1.61 Mb。

关 键 词:甘蓝  抽薹  遗传分析  QTL定位  

Genetic and QTL Mapping Analysis of Bolting Time in Cabbage(Brassica oleracea)
WANG Wuhong,WANG Jinglei,LI Biyuan,WEI Qingzhen,HU Tianhua,HU Haijiao,BAO Chonglai.Genetic and QTL Mapping Analysis of Bolting Time in Cabbage(Brassica oleracea)[J].Acta Horticulturae Sinica,2020,47(5):974-982.
Authors:WANG Wuhong  WANG Jinglei  LI Biyuan  WEI Qingzhen  HU Tianhua  HU Haijiao  BAO Chonglai
Institution:Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
Abstract:We constructed the populations of F1,BC1,BC2,and F2 by using cabbage lines‘S-1’(early flower)and‘G-1’(late flower)as parents and adopted the plant quantitative trait major gene + polygenic mixed genetic model method to analysis the genetic model of bolting time of cabbage. Besides,we used SLAF-BSA technology to mapping the bolting time QTLs. The results showed that the bolting time fitted the model of two additive-dominant-epistatic major genes and additive-dominance polygenes. The average heritability of major genes plus polygenes was 93.41%,which indicated that bolting trait of cabbage was mainly affected by major gene inheritance. Moreover,two QTLs were detected,which located on chromosome C02 2.31–3.09 Mb and 33.57–34.40 Mb,respectively,with a total length of 1.61 Mb.
Keywords:cabbage  Brassica oleracea  bolting  genetic analysis  QTL mapping  
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