| 外源乙烯对草莓果实微粒体Ca2+- ATPase活性和膜脂过氧化的调节 |
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| 引用本文： | 樊秀彩,关军锋,刘崇怀,张继澍,李广敏.外源乙烯对草莓果实微粒体Ca2+- ATPase活性和膜脂过氧化的调节[J].园艺学报,2004,31(2):227-229. |
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| 作者姓名： | 樊秀彩 关军锋 刘崇怀 张继澍 李广敏 |
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| 作者单位： | (中国农业科学院郑州果树研究所，郑州 450009； 河北省农林科学院遗传生理研究所，石家庄 050051； 西北农林科技大学生命科学学院，杨凌 712100) |
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| 基金项目： | 国家自然科学基金资助项目(30270933) |
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| 摘 要： | 以乳白期‘春星’草莓(Fragaria ananassa Duch．‘Chunxing’)果实为试材，研究了经钙调素(CaM)拮抗剂三氟拉嗪(TFP)、氯丙嗪(CPZ)各100 µmol·L-1 和钙通道阻塞剂异博定(Verapamil)100 µmol·L-1 预处理后再用乙烯(50 µL·L-1 )处理的草莓果实中微粒体“Ca2+-ATP酶活性、O2 产生速率和MDA含量的变化。结果表明，外源乙烯对微粒体膜O2 产生速率无显著影响，处理早期提高微粒体膜Ca2+- ATPase总活性，对MDA含量影响不大；后期加速微粒体膜Ca2+- ATPase总活性下降，但仍保持较高的MDA含量和线粒体膜Ca2+-ATPase活性。CPZ、TFP和Verapamil预处理降低了上述乙烯处理下Ca2+- ATPase活性和MDA含量，但对微粒体膜O 产生速率亦无显著影响，这说明细胞内Ca2+-和CaM 可能参与了乙烯诱导的膜Ca2+-ATP酶活性与膜脂过氧化水平的调节。
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| 关 键 词： | 草莓 果实 微粒体 乙烯 钙离子-ATP酶活性 膜脂过氧化 丙二醛 钙信使系统 钙调素 |
| 文章编号： | 0513-353X(2004)02-0227-03 |
| 收稿时间： | 2003-5-22 |
| 修稿时间： | 2003-8-20 |
Regulation of Exogenous Ethylene on Ca2+-ATPase Activity and Lipid Peroxidation of Microsomal Membrane in Strawberry Fruits |
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| Fan Xiucai,Guan Junfeng,Liu Chonghuai,Zhang Jishu,and Li Guangmin.Regulation of Exogenous Ethylene on Ca2+-ATPase Activity and Lipid Peroxidation of Microsomal Membrane in Strawberry Fruits[J].Acta Horticulturae Sinica,2004,31(2):227-229. |
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| Authors: | Fan Xiucai Guan Junfeng Liu Chonghuai Zhang Jishu and Li Guangmin |
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| Institution: | ( Zhengzhou Fruit Research Insthute，Chinese Academy of Agricultural Sciences，Zhengzhou 450009，China； Insthute of Genetics and Physiology，Hebei Academy of Agricultural and Forestry Sciences，Shijiazhuang 050051，China； College of Life Science，Northwest Sci-Tech University of Agriculture and Forestry，Yangling 712100，China) |
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| Abstract: | The strawberry fruits(Fragaria ananassa Duch．‘Chunxing’) harvested at white stage were used to investigate the efects of exogenous ethylene (50 µL·L-1 ) on Ca2+-ATPase activities，O production rate and MDA content of microsomal membrane with or without pretreatment of 100 µmol·L-1 chloropromaize(CPZ)，trifluoperizine(TFP)and verapamil(VER)，respectively，The results showed that the exogenous ethylene had no obvious influence on O production rate，and had no much effect on MDA content，but stimulated Ca2+-ATPase activitv of microsomal membrane at 12 h after ethylene treatment．Whereas a decreasing of Ca2+-ATPase activities， and relatively higher MDA content were found at 24 h after ethylene removal．The pretreatment of CPZ,TFP and VER reduced the Ca2+-ATPase activities and MDA content of microsomal memb rane under ethylene treatment， but had no significant efect on O production rate．These suggested that cellular Ca2+-CaM may be involved in regulation of Ca2+-ATPase activity and lipid peroxidation of microsomal membrane induced by ethylene． |
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| Keywords: | Strawberry Ethylene Calcium messenger system Fruit Membrane Ca2 -ATPase Lipid peroxidation |
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