| 白莲腐败病病原菌的分离鉴定 |
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| 引用本文： | 胡长志.白莲腐败病病原菌的分离鉴定[J].长江蔬菜,2013(18):92-94. |
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| 作者姓名： | 胡长志 |
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| 作者单位： | [1]江西农业大学农学院,南昌330045 [2]江西广昌白莲产业发展局,南昌330045 |
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| 基金项目： | 国家自然科学基金（31301620）,江西省科技计划项目（20121BBF60049）,江西省教育厅项目（GJJ12247） |
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| 摘 要： | 莲腐败病是白莲种植区发生最普遍、为害最严重的病害之一。本试验对发病白莲上的病原茵进行分离鉴定。该病原分离物在PSA培养基上气生茵丝由白色渐变成深紫色，并产生大小两种孢子。大型分生孢子镰刀形，无色，稍呈弯曲，两端尖细，大小为（39．1-55．2）μm×（2．8～4．6）p,m，多为3个隔膜，少部分为1-2隔或4~5隔；小型分生孢子椭圆形。无色，无隔膜，大小为（5．0～9．2）μm×（2．0～3．5）μm。致病性测定表明分离物能100％引起白莲出现典型的白莲腐败病的症状。ITSl／ITS4的PCR扩增产物与GenBank中Fusariumoxysporum￡sp．nelumbicola的序列同源性为99％。研究结果表明。分离到的病原物为尖孢镰刀茵莲专化型Foxysporum f.sp．ndumbicola。
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| 关 键 词： | 莲腐败病 尖孢镰刀菌莲专化型 鉴定 |
Isolation and Identification of Pathogens Causing Rhizome Rot of Lotus |
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| HU Changzhi,SUN Xiaotang,XIE Keqiang,JIANG Junxi,ZHENG Xingwen,CUI Ruqiang.Isolation and Identification of Pathogens Causing Rhizome Rot of Lotus[J].Journal of Changjiang Vegetables,2013(18):92-94. |
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| Authors: | HU Changzhi SUN Xiaotang XIE Keqiang JIANG Junxi ZHENG Xingwen CUI Ruqiang |
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| Institution: | 1 ( 1.College of Agronomy, Jiangxi Agffeuhural University, Nanehang 330045; 2.Guangehang Development Bureau of White Lotus Industry ) |
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| Abstract: | Rhizome rot was the most common disease of lotus. We isolated six isolates from diseased roots of lotus from Guangchang county of Jiangxi province in July 2011, and identified the isolates through their morphological and molecular characteristics. On PSA medium, the aerial mycelium was white firstly, and then changed to dark purple. Their macroconidia were fusiform, slightly curved, pointed at two ends, （39.1-55.2）μm×（2.8-4.6） μm in size, and mostly had three septa. Microconidia were colourless, ellipsoidal or cylindrical, never in chains, mostly non-septate, straight or curved, with the size of （5.0-9.2） μm×（2.0-3.5） μm. The typical symptoms of rhizome rot occurred on all louts seedlings infected with the isolates. To verify the pathogen, we sequenced rDNA-ITS fragments of the isolates amplified by primers ITS1 and ITS4, and 99% of sequences were homologous with those of Fusaffum oxysporum f. sp. nelumbicola in GenBank. The above results showed the isolates were F. oxysporum f. sp. nelumbicola. |
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| Keywords: | Rhizome rot of lotus Fusadum oxysporum f sp nelumbicola Identification |
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