| 小白菜自交不亲和性相关基因的分子标记开发 |
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| 引用本文： | 贾永鹏,李霞,周国林,林处发,汪爱华.小白菜自交不亲和性相关基因的分子标记开发[J].长江蔬菜,2016(8):38-41. |
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| 作者姓名： | 贾永鹏 李霞 周国林 林处发 汪爱华 |
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| 作者单位： | 1. 武汉市农科院蔬菜科学研究所，340065; 华中农业大学;2. 武汉市农科院蔬菜科学研究所,340065;3. 武汉市农科院 |
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| 基金项目： | “十二五”国家科技支撑计划项目资助(2012BAD02B01-17)；武汉市农科院2013年英才计划项目 |
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| 摘 要： | 以自交亲和系WS-199和自交不亲和系WS-85为亲本杂交获得的F_2分离群体为供试材料,采用BSA法结合SNP芯片技术,筛选出与自交不亲和性相关的SNP标记,并将SNP差异位点序列信息与白菜基因组序列进行比对分析,并进一步开发SSR标记,共获得了与自交不亲和性相关的SSR分子标记BrA1-2、BrA1-3和BrA1-14,为分子标记辅助自交不亲和性杂交种生产提供技术支持。
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| 关 键 词： | 小白菜 自交不亲和性 SNP 分子标记 |
Molecular Marker Development of Self-incompatibility Related Genes in Chinese Cabbage |
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| JIA Yongpeng,LI Xia,ZHOU Guolin,LIN Chufa,WANG Aihua.Molecular Marker Development of Self-incompatibility Related Genes in Chinese Cabbage[J].Journal of Changjiang Vegetables,2016(8):38-41. |
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| Authors: | JIA Yongpeng LI Xia ZHOU Guolin LIN Chufa WANG Aihua |
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| Abstract: | Taking an F2 segregating population obtained by hybridization between self-compatible line WS-199 and self-incompatible line WS-85 as materials, combining with the BSA method and SNP biochip technology, we screened SNP markers which related to self-incompatibility, and compared the sequence information of SNP different loci with cabbage genome sequences. Furtherly, we developed SSR markers, and got SSR molecular markers BrA1-2, BrA1-3 and BrA1-14 which were related to self-incompatibility, providing technical support for the production of self-incompatibility hybrid assisted by molecular markers. |
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| Keywords: | Chinese cabbage Self incompatibility SNP Molecular markers |
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