| 苦苣菜再生体系建立研究 |
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| 引用本文： | 于颖,何漫,吴冰冰,徐娜,姜长阳.苦苣菜再生体系建立研究[J].中国园艺文摘,2009,25(11):20-22. |
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| 作者姓名： | 于颖 何漫 吴冰冰 徐娜 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 基金项目： | 辽宁师范大学教学改革项目 |
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| 摘 要： | 以苦苣菜的嫩茎为材料，进行愈伤组织的诱导、分化、试管苗生根、移栽及移植所需要条件的研究，并建立起苦苣菜嫩茎的再生体系。结果证明：MS＋BA0．6mg／L＋2，4-D1．6mg／L是嫩茎颗粒状愈伤组织诱导的理想培养基；MS＋AgNO3 2．0mg／L＋BA1．0mg／L＋NAA0．1mg／L是诱导愈伤组织颗粒和不定茅分化培养的理想培养基；1／2MS＋IAA0．2mg／L＋NAA0．1mg／L是试管苗生根培养的理想培养基；河沙和炉灰渣是试管苗移栽的理想基质，移栽成活率为96％和98．5％，移植到山坡上的试管苗生长旺盛，根系发达。
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| 关 键 词： | 苦苣菜 愈伤组织 再生体系 |
Research on Establishing the Regeneration System of Sonchus oleraceus L. |
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| Authors: | YU Ying HE Man WU Bing-bing XU Na JIANG Chang-yang |
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| Institution: | YU Ying HE Man WU Bing-bing XU Na JIANG Chang-yang |
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| Abstract: | Sonchus oleraceus L. was used as materials to make research on callus induction and differentiation, taking roots, transplanting of test-tube plantlets and also its conditions. The regeneration system was established successfully. The results indicated that MS＋BA0.6 mg/L＋2,4-D1.6mg/L was ideal medium for inducing graininess callus of tender stem; MS＋AgNO32.0 rag/L＋ BA1.0 mg/L＋NAA0.1 mg/L was ideal medium for differentiating adventitious buds and 1/2 MS＋ IAA0.2mg/L＋NAA0. lmg/L was best concentration for taking roots. The test-tube plantlets transplanted to hillside could grow well with the ideal groundmass-sands and cinder. The survi,Cal ratio was 96% and 98.5% respectively. |
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| Keywords: | Sonchus oleraceus callus regeneration system |
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