Evaluation of Intestinal Absorption of Dietary Halocynthiaxanthin,a Carotenoid from the Sea Squirt Halocynthia roretzi |
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| Authors: | Chiaki Ikeda Yuki Manabe Nami Tomonaga Tatsuya Wada Takashi Maoka Tatsuya Sugawara |
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| Institution: | 1.Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan; (C.I.); (Y.M.); (N.T.);2.Nihon Pharmaceutical Co., Ltd., Tokyo 1030012, Japan;3.Division of Food Function and Chemistry, Research Institute for Production Development, Kyoto 6060805, Japan; |
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| Abstract: | Halocynthiaxanthin is an acetylenic carotenoid mainly found in Halocynthia roretzi. To date, several bioactivities of halocynthiaxanthin have been reported, but its mechanism of digestion and absorption in mammals has not been studied yet. In this study, we evaluated the intestinal absorption of halocynthiaxanthin in mice. The halocynthiaxanthin-rich fraction was prepared from the tunicate Halocynthia roretzi. Mice were orally administered the fraction at a dose of 5 mg/kg body weight. The halocynthiaxanthin levels in the plasma, liver, and small intestine, were quantified using HPLC-PDA, 1, 3, 6, and 9 h after ingestion. The halocynthiaxanthin-rich fraction mainly consisted of the all-trans form and a small amount of cis forms. These three isomers were detected in the plasma of mice 3 h after ingestion. Time-course changes after the ingestion of this fraction were found, with cis isomers being more abundant than the all-trans isomer in the mouse plasma and liver. In the small intestine, however, the all-trans isomer was primarily detected. The possibility that cis isomers might be absorbed rapidly from the small intestine cannot be denied, but our results suggest that dietary all-trans-halocynthiaxanthin might be isomerized to the cis isomer after intestinal absorption. |
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| Keywords: | halocynthiaxanthin intestinal absorption carotenoid functional food metabolism |
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