| 3个甘蔗与斑茅远缘杂交后代BC1的染色体遗传分析 |
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| 引用本文： | 黄永吉,符成,林炜乐,刘少谋,高嘉慧,邓祖湖,黄忠兴,林彦铨,陈如凯.3个甘蔗与斑茅远缘杂交后代BC1的染色体遗传分析[J].热带作物学报,2015,36(1):53-58. |
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| 作者姓名： | 黄永吉 符成 林炜乐 刘少谋 高嘉慧 邓祖湖 黄忠兴 林彦铨 陈如凯 |
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| 作者单位： | 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室;广州甘蔗糖业研究所;福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室;广州甘蔗糖业研究所;福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室;福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室;广州甘蔗糖业研究所;福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室;福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 |
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| 基金项目： | 国家自然科学基金（No. 30671329）；国家甘蔗产业体系专项资金资助（No. CARS-20-1-5）。 |
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| 摘 要： | 对3个甘蔗与斑茅远缘杂交后代BC1进行真实性鉴定及染色体核型分析,以探讨甘蔗与斑茅BC1的染色体传递方式。利用2对鉴定斑茅真实杂交后代的特异引物对3个甘蔗与斑茅BC1进行鉴定,采用根尖分生区细胞去壁低渗涂片法制片,显微拍照计数染色体数目,并进行染色体核型分析。3个BC1材料均为斑茅的真实杂交后代,崖城01-69体细胞染色体核型公式为2n=121=120 m+1 sm,其染色体按2n+n方式传递;崖城01-116的体细胞染色体核型公式为2n=122=118 m+4 sm,其染色体传递方式为2n+n;崖城01-134的体细胞染色体核型公式为2n=121=120 m+1 sm,其染色体传递为2n+n。推断甘蔗与斑茅BC1的染色体以2n+n的方式传递。
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| 关 键 词： | 甘蔗 斑茅 核型分析 染色体传递方式 |
Genetic Analysis of Chromosome in 3 BC1 Clones from the Distant Crossing Between Saccharum spp. and Erianthus arundinaceus |
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| HUANG Yongji,FU Cheng,LIN Weile,LIU Shaomou,GAO Jiahui,DENG Zuhu,HUANG Zhongxing,LIN Yanquan and CHEN Rukai.Genetic Analysis of Chromosome in 3 BC1 Clones from the Distant Crossing Between Saccharum spp. and Erianthus arundinaceus[J].Chinese Journal of Tropical Crops,2015,36(1):53-58. |
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| Authors: | HUANG Yongji FU Cheng LIN Weile LIU Shaomou GAO Jiahui DENG Zuhu HUANG Zhongxing LIN Yanquan and CHEN Rukai |
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| Institution: | Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture;Guangzhou Research Institute for Sugarcane Industry;Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture;Guangzhou Research Institute for Sugarcane Industry;Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture;Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture;Guangzhou Research Institute for Sugarcane Industry;Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture;Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture |
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| Abstract: | Circumstance of rice virus disease in southern Hainan island rice-planting areas including Sanya, Qionghai and Lingshui was investigated. Specific primers for 10 known rice virus species were designed for PCR tests. A total of 23 suspected virus-infected samples were tested. Molecular identification results showed that there were two major rice virus species existed in Hainan winter-breeding areas: southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV). 6 SRBSDV-infected samples and 14 RRSV-infected samples were identified by PCR and sequencing methods. 2 samples were co-infected by both SRBSDV and RRSV. Infection rates of SRBSDV and RRSV were 26.09% and 60.87%, separately. The other 8 viruses were not detected. This study showed that SRBSDV and RRSV are key diseases in Hainan winter-breeding areas and should be carefully prevented. |
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| Keywords: | Sugarcane Erianthus arundinaceus Karyotype analysis Chromosome transmission |
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