| 香蕉MaWRKY1转录因子在果实和幼苗诱导抗冷性中表达分析 |
| |
| 引用本文： | 洪克前,谷会,陈丽.香蕉MaWRKY1转录因子在果实和幼苗诱导抗冷性中表达分析[J].热带作物学报,2021,42(2):303-309. |
| |
| 作者姓名： | 洪克前 谷会 陈丽 |
| |
| 作者单位： | 中国热带农业科学院南亚热带作物研究所/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 524091 |
| |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项——中国热带农业科学院院级创新团队项目(No.1630062017032,No.1630062017031)。 |
| |
| 摘 要： | 为了探究香蕉MaWRKY1转录因子在抗冷诱导过程中的作用,采用外源过氧化氢（H2O2）、茉莉酸甲酯（MeJA）、水杨酸（SA）和丙烯（propylene）等处理香蕉果实,同时采用外源H2O2、MeJA和脱落酸（ABA）等处理香蕉幼苗,处理结束后,将实验材料放置于7 ℃下贮藏,通过Northern blot技术分析实验材料中MaWRKY1基因表达变化。H2O2、MeJA、SA和propylene等处理均使果实冷害症状推迟2 d出现;处理组果实中MaWRKY1 mRNA积累均早且高于对照组。H2O2、MeJA和ABA等处理也均使幼苗推迟18 h显现冷害症状;处理组幼苗中MaWRKY1基因表达提前且表达量相对较高。相比较这几种外源性调节剂,MeJA和H2O2处理的香蕉果实和幼苗所获得的抗冷效果优于其他生长调节剂,同时MaWRKY1基因表达水平也比其他处理组提前或表达量更高,推测MaWRKY1可能更加积极响应MeJA或H2O2诱导香蕉耐冷性
|
| 关 键 词： | 香蕉 WRKY1 转录因子 耐冷性 |
| 收稿时间： | 2020-01-20 |
Expression Analysis of MaWRKY1 Factor in Inducing Chilling Resistance of Banana Fruits and Seedlings |
| |
| HONG Keqian,GU Hui,CHEN Li.Expression Analysis of MaWRKY1 Factor in Inducing Chilling Resistance of Banana Fruits and Seedlings[J].Chinese Journal of Tropical Crops,2021,42(2):303-309. |
| |
| Authors: | HONG Keqian GU Hui CHEN Li |
| |
| Institution: | South Subtropical Crops Research Institutes, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory for Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, Zhanjiang, Guangdong 524091, China |
| |
| Abstract: | To investigate the role of MaWRKY1 in induction of chilling resistance of the banana,fruits were treated with H2O2,MeJA,SA and propylene,respectively,and seedlings were treated with H2O2,MeJA and ABA,respectively.Following treatments,fruits and seedlings were stored at 7℃.Northern blot technique was adopted to analyze the changes of MaWRKY1 in fruits and seedlings inducted by chilling resistance.The results indicated that H2O2,MeJA,SA,and propylene treatments delayed the occurrence of chilling injury symptom of fruits by 2 d at 7℃.MaWRKY1 mRNA accumulation in the treated fruits was earlier and higher than that of the untreated.The treatment of H2O2,MeJA,and ABA retarded the occurrence of chilling injury symptom of seedlings by 18 h at 7℃.In addition,MaWRKY1 mRNA accumulation level was significantly earlier and higher in the treated seedlings than that of the untreated.Compared with other exogenous growth regulators,fruits and seedlings treated with MeJA,and H2O2,respectively had a better chilling resistance,and the MaWRKY1 gene expression was earlier or higher than that in other treated groups,MaWRKY1 might be more active in chilling resistance of banana fruits and seedlings inducted by MeJA or H2O2. |
| |
| Keywords: | |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
