| 外源NO对低温胁迫下‘胭脂茄’种子萌发及幼苗生理特性的影响 |
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| 引用本文： | 陈瑶瑶,严良文,刘智成,郑作芸,余洁,陈萍萍,江巍.外源NO对低温胁迫下‘胭脂茄’种子萌发及幼苗生理特性的影响[J].热带作物学报,2022,43(7):1477-1484. |
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| 作者姓名： | 陈瑶瑶 严良文 刘智成 郑作芸 余洁 陈萍萍 江巍 |
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| 作者单位： | 1.龙岩市农业科学研究所,福建龙岩 3640002. 龙岩市新罗区农业局,福建龙岩 364000 |
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| 基金项目： | 龙岩市科技项目(No.2019LYF12011)； |
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| 摘 要： | 以闽西地方茄子品种‘胭脂茄’种子和幼苗为试材,用不同浓度SNP（0、0.1、0.2、0.3、0.4、0.5、1.0、1.5 mmol/L）浸种6 h,分别在28℃和4℃暗培养处理3 d后转入培养箱进行常规培养萌发,统计各处理的发芽指标;对胭脂茄幼苗叶面喷施不同浓度SNP（0、0.1、0.3、0.5、0.8、1.0、1.2、1.5 mmol/L）预处理3 d后,于10℃/5℃下进行低温胁迫,通过测定幼苗的株高、茎粗、鲜重、干重及叶片中超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）的活性,可溶性蛋白和脯氨酸含量以及丙二醛（MDA）含量,研究低温胁迫下外源一氧化氮（NO）预处理后对种子萌发、幼苗生长及相关生理生化特征的影响。结果表明,外源NO供体SNP预处理后,胭脂茄种子的发芽率、发芽势、发芽指数和活力指数的变化趋势均随着SNP浓度的升高呈先增加后减少变化,SNP浓度为0.2 mmol/L时,种子萌发各指标达到最大,SNP浓度超过0.5 mmol/L时各指标降低;施加低浓度（0.1~1.0 mmol/L）SNP预处理能够增加胭脂茄幼苗株高、茎粗、鲜重和干重,可以增加幼苗叶片中可溶性蛋白和脯氨酸含量、提高超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性,降低幼苗叶片中MDA累积,SNP浓度为0.5 mmol/L时CAT活性最高,其余各项指标以SNP浓度为0.8 mmol/L时达到最佳,高浓度SNP对各项指标产生抑制作用。研究结果表明低温胁迫下低浓度SNP预处理后可有效保护胭脂茄种子萌发和幼苗生长,浓度过高反而造成伤害。本研究结果为合理使用外源NO促进胭脂茄种子萌发和幼苗生长提供理论依据,为闽西地区有效防御茄子低温冷害提供应用价值。
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| 关 键 词： | 外源NO 低温胁迫 胭脂茄 种子萌发 生理特性 |
| 收稿时间： | 2021-12-21 |
Effect of Exogenous NO on the Germination of Eggplant (Solanum melongena cv. Yanzhiqie) Seeds and the Physiological Characteristics of Seedlings under Low Temperature Stress |
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| CHEN Yaoyao,YAN Liangwen,LIU Zhicheng,ZHENG Zuoyun,YU Jie,CHEN Pingping,JIANG Wei.Effect of Exogenous NO on the Germination of Eggplant (Solanum melongena cv. Yanzhiqie) Seeds and the Physiological Characteristics of Seedlings under Low Temperature Stress[J].Chinese Journal of Tropical Crops,2022,43(7):1477-1484. |
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| Authors: | CHEN Yaoyao YAN Liangwen LIU Zhicheng ZHENG Zuoyun YU Jie CHEN Pingping JIANG Wei |
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| Institution: | 1. Longyan Institute of Agricultural Sciences, Longyan, Fujian 364000, China2. Agriculture Bureau of Xinluo District, Longyan, Fujian 364000, China |
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| Abstract: | Seeds and seedlings of Solanum melongena cv. Yanzhiqie, a local eggplant cultivar in western Fujian, were soaked with different concentrations of (0, 0.1, 0.2, 0.3, 0.4, 0.5, 1.0, 1.5 mmol/L) for 6 hours, respectively cultured in dark at 28℃ and 4℃ for 3 days and then transferred to an incubator for conventional culture and germination, and the germination indicators of each treatment were statistically analyzed. The leaves of S. melongena cv. Yanzhiqie seedlings were pretreated with different concentrations of SNP (0, 0.1, 0.3, 0.5, 0.8, 1.0, 1.2, 1.5 mmol/L) for 3 days and subjected to low temperature stress at 10℃ / 5℃. The effects of exogenous nitric oxide (NO) pretreatment on the germination of seeds, and growth and related physiological and biochemical characteristics of seedlings were studied by determining plant height, stem diameter, fresh weight, and dry weight of seedlings, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and contents of soluble protein, proline, and malondialdehyde (MDA) in leaves. The germination percentage, germination energy, germination index and vigor index of S. melongena cv. Yanzhiqie seeds increased firstly and then decreased with the increase of SNP (an exogenous NO donor) concentration, and all indicators of seed germination reached the maximum at 0.2 mmol/L, and decreased at > 0.5 mmol/L.Pretreatment with low concentration (0.1-1.0 mmol/L) of SNP could increase plant height, stem diameter, fresh weight and dry weight of S. melongena cv. Yanzhiqie seedlings, increase the contents of soluble protein and proline in leaves, improve the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and decrease MDA accumulation in leaves, The CAT activity was the highest at 0.5 mmol/L, and other indicators were optimal at 0.8 mmol/L. High concentration of SNP inhibited all indicators. The above suggests that pretreatment with low concentration of SNP could effectively promote the germination of S. melongena cv. Yanzhiqie seeds and growth of seedlings under low temperature stress, while high concentration cause damage. The results of this study would provide a theoretical basis for the proper use of exogenous NO to promote the germination of S. melongena cv. Yanzhiqie seeds and the growth of seedlings, and are of reference value for effectively protecting eggplants from low temperature damage in western Fujian. |
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| Keywords: | exogenous NO low temperature stress Solanum melongena cv Yanzhiqie seed germination physiological characteristics |
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