| 强化科研服务理念 提升科研服务质量 |
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| 引用本文： | 刘 诚,郑少强,陈展雄,李建辉.强化科研服务理念 提升科研服务质量[J].热带作物学报,2013,33(5):43-45. |
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| 作者姓名： | 刘 诚 郑少强 陈展雄 李建辉 |
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| 作者单位： | 1 海南大学农学院 海南海口 570228;1 海南大学农学院 海南海口 570228;2 中国热带农业科学院热带作物品种资源研究所 海南儋州 571737;3 海南省琼州学院生物科学与技术学院 海南三亚 572022 |
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| 摘 要： | 以秋水仙素诱导获得的鹤顶兰嵌合体无菌苗的假鳞茎、茎尖及除根苗为外植体，分别接种于花宝一号的固体、半固体及固液双层培养基上进行培养，通过诱导类原球茎的方法对嵌合体进行分离。结果表明，鹤顶兰嵌合体分离最佳方法为将除根苗插入花宝一号＋100.0 ml/L椰子水＋ 2.0 g/L活性炭＋ 20.0 g/L蔗糖＋2.0 mg/LBA＋0.2 mg/LNAA的半固体培养基上，诱导出PLB进而分化成苗，分离得到纯合四倍体。
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| 关 键 词： | 鹤顶兰 嵌合体 组织培养 四倍体 |
Strengthen the Scientific Research Service Concept to Improve the Scientific Research Service Quality |
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| LIU Cheng,ZHENG Shaoqiang,CHEN Zhanxiong and LI Jianhui.Strengthen the Scientific Research Service Concept to Improve the Scientific Research Service Quality[J].Chinese Journal of Tropical Crops,2013,33(5):43-45. |
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| Authors: | LIU Cheng ZHENG Shaoqiang CHEN Zhanxiong and LI Jianhui |
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| Institution: | 1 College of Agriculture, Hainan University, Haikou, Hainan 570228;1 College of Agriculture, Hainan University, Haikou, Hainan 570228;2 Tropical Crops Genetic Resources Institute, CATAS, Danzhou, Hainan 571737;;3 School of Biological Science and Technology, Qiongzhou University, Sanya, Hainan 572022 |
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| Abstract: | In order to obtain pure teraploid of Phaius tankervilleae, improve polyploidy induction efficiency. Through inducting protocorm like body for separation of chimera, the research used the pseudobulb, root segment, shoot apex as explants cultured with Hyponex No.1, solid, semi-solid and liquid-solid double-layer medium. The results showed that the best method of inducting protocorm like body was inserting the resection of the plant root to semi-solid Hyponex No.1 + 100 ml/L CW + 2 g/L AC + 20 g/L sucrose + 2.0 mg/L BA + 0.2 mg/LNAA. Two pure teraploid were obtained from this. The method can be used to separate Phaius tankervilleae chimera. |
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| Keywords: | Phaius tankervilleae chimera tissue culture tetraploid |
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