| 油菜素内酯应用于甘蔗组织培养快繁的研究 |
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| 引用本文： | 张娅,顾明华,朱可针,梁琼月,龚银花,何冰,何丽珊.油菜素内酯应用于甘蔗组织培养快繁的研究[J].热带作物学报,2021,42(12):3486-3491. |
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| 作者姓名： | 张娅 顾明华 朱可针 梁琼月 龚银花 何冰 何丽珊 |
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| 作者单位： | 1.广西大学农学院,广西南宁 5300042.广西甘蔗生物学重点实验室,广西南宁 5300043.广西农业环境与农产品安全重点实验室,广西南宁 5300044.植物科学国家级实验教学示范中心（广西大学）,广西南宁 5300045.广西展欣农业科技股份有限公司,广西南宁 530004 |
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| 基金项目： | 国家自然科学基金青年科学基金项目(31601235);广西自然科学基金青年基金项目(2017GXNSFBA198241);博世科青年教师创新人才培养基金项目(BRP180251) |
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| 摘 要： | 为了研究油菜素内酯（brassinolide, BL）对甘蔗组织培养的影响,本研究以“植物器官从头再生”“体细胞胚再生”2种组培快繁体系为基础,通过添加适宜浓度的2,4-表油菜素内酯（2,4-epibrassinolide, 2,4-EBL）于基础培养基中,设计了6套适用于甘蔗快繁的组合培养基方案。并于不同诱导阶段（不定根诱导、愈伤组织诱导、成苗诱导）对接种材料的形态特征及内源植物激素（油菜素内酯、玉米素核苷、生长素、脱落酸）的含量变化进行了统计与测定,探讨了油菜素内酯应用于甘蔗组织培养快繁技术的可行性。结果表明,将不定根培养基MS + 0.2 mg/L 2,4-D + 0.2 mg/L LFS与成苗培养基MS + 0.5 mg/L KT +（0.2 mg/L IBA / 0.2 mg/L 2,4-EBL）+ 300 mg/L Pro进行组合,为甘蔗组培快繁的较优方案,油菜素内酯可应用于甘蔗组织培养快繁技术,且体细胞胚再生成苗优于愈伤组织再生成苗,成苗时间可缩短至32 d。
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| 关 键 词： | 甘蔗 组织培养 油菜素内酯 植物激素 |
| 收稿时间： | 2021-02-18 |
Application of Brassinolide in the Rapid Propagation of Sugarcane Tissue Culture |
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| ZHANG Ya,GU Minghua,ZHU Kezhen,LIANG Qiongyue,GONG Yinhua,HE Bing,HE Lishan.Application of Brassinolide in the Rapid Propagation of Sugarcane Tissue Culture[J].Chinese Journal of Tropical Crops,2021,42(12):3486-3491. |
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| Authors: | ZHANG Ya GU Minghua ZHU Kezhen LIANG Qiongyue GONG Yinhua HE Bing HE Lishan |
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| Institution: | 1. College of Agriculture, Guangxi University, Nanning, Guangxi 53004, China2. Guangxi Key Laboratory of Sugarcane Biology, Nanning, Guangxi 53004, China3. Key Laboratory of (Guang Xi) Agriculture Environment and Products Safety, Nanning, Guangxi 53004, China4. National Demonstration Center for Experimental Plant Science Education (Guangxi University), Nanning, Guangxi 53004, China5. Guangxi Zhanxin Agricultural Technology Co., Ltd., Nanning, Guangxi 53004, China |
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| Abstract: | Based on two tissue culture and rapid propagation systems of “plant organ de novo regeneration” and “somatic embryo regeneration”, six combinations of medium were designed by adding appropriate concentration of 2,4-epibrassinolide into the basic medium to explore the effects of brassinolide (BL) on sugarcane tissue culture. The morphological characteristics of different induction stages, including adventitious root induction, callus induction and seedling induction, were analyzed. The content changes of endogenous plant hormones (brassinolide, zeatin riboside, indole 3 acetic acid, abscisic acid) were determined. The feasibility of brassinolide application in sugarcane tissue culture and rapid propagation was discussed. The combination of adventitious root medium MS+0.2 mg/L 2.4-D+0.2 mg/L LFS and seedling medium MS + 0.5 mg/L KT+(0.2 mg/L IBA / 0.2 mg/L 2,4-EBL)+ 300 mg/L Pro was the better scheme for sugarcane tissue culture and rapid propagation. Brassinolide could be used in tissue culture and rapid propagation of sugarcane. The regeneration time of somatic embryo was better than that of callus, which could be shortened to 32 days. |
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| Keywords: | sugarcane tissue culture brassinolide phytohormone |
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