| 花前氮亏缺对水稻叶片氮代谢酶活性的影响 |
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| 引用本文： | 武辉,向镜,陈惠哲,张玉屏,张义凯,姬广梅,王亚梁,史鸿志,朱德峰.花前氮亏缺对水稻叶片氮代谢酶活性的影响[J].中国稻米,2017(4):14-19. |
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| 作者姓名： | 武辉 向镜 陈惠哲 张玉屏 张义凯 姬广梅 王亚梁 史鸿志 朱德峰 |
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| 作者单位： | 中国水稻研究所/水稻生物学国家重点实验室,杭州,310006 |
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| 基金项目： | 粮丰工程项目(2016YFD0300502),国家自然科学基金青年科学基金项目(31501272),2015年浙江省"三农六方"项目 |
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| 摘 要： | 以籼型杂交稻中浙优1号和籼粳型杂交稻甬优12为材料,试验分析了花前不同时期氮亏缺处理对水稻叶片氮代谢酶活性的影响。结果表明,花前氮亏缺导致植株上3叶氮浓度、NR、GS、GOGAT、GOT和GPT酶活性大幅下降,GDH活性显著增长,且各叶位对花前氮亏缺敏感度总体上表现为剑叶倒2叶倒3叶;破口期亏氮对籼型杂交稻中浙优1号上3叶氮同化酶活性的影响远小于减数分裂期处理,与之相比,籼粳杂交稻甬优12对破口期亏氮胁迫仍较敏感,表明中浙优1号植株氮代谢酶的亏氮敏感性由减数分裂期至破口期逐步下降,对土壤速效氮的需求同步降低,甬优12则对土壤供氮存在更高需求。
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| 关 键 词： | 水稻 氮亏缺 花前 氮代谢 减数分裂期 破口期 |
Effects of Pre-anthesis Nitrogen Deficiency on Nitrogen Metabolism Enzyme Activities of Rice Leaves |
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| WU Hui,XIANG Jing,CHEN Huizhe,ZHANG Yuping,ZHANG Yikai,JI Guangmei,WANG Yaliang,SHI Hongzhi,ZHU Defeng.Effects of Pre-anthesis Nitrogen Deficiency on Nitrogen Metabolism Enzyme Activities of Rice Leaves[J].China Rice,2017(4):14-19. |
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| Authors: | WU Hui XIANG Jing CHEN Huizhe ZHANG Yuping ZHANG Yikai JI Guangmei WANG Yaliang SHI Hongzhi ZHU Defeng |
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| Abstract: | Effects of pre-anthesis nitrogen deficiency at different growth stages on nitrogen metabolism enzyme activities in main func-tional leaves were studied, using indica-japanica hybirid rice variety Yongyou 12, and indica hybrid rice variety Zhongzheyou 1 as materials. The results showed that, affected by nitrogen deficiency at meiosis stage and rupturing stage, nitrogen concentration, NR, GS, GOGAT, GOT and GPT enzyme activities of top-three leaves were dramatically decreased, but GDH activity was increased signifi-cantly. In general, the sensitivity of enzyme activities of leaves at different leaf positions to nitrogen deficiency were enhanced from bottom to top. Furthermore, the effects of nitrogen deficiency at rupturing stage on nitrogen assimilation enzyme activities of indica hy-brid rice Zhongzheyou1 was much less than that of meiosis stage. However, indica-japanica hybirid rice Yongyou 12 was still sensitive to nitrogen deficiency during rupturing stage. This indicated that the nitrogen deficiency sensitivity of nitrogen metabolism enzymes in Zhongzheyou 1 plants decreased gradually from meiosis stage to rupturing stage, and the demand for soil available nitrogen was de-creased synchronously, while Yongyou 12 had higher demand for soil nitrogen supply. |
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| Keywords: | rice nitrogen deficiency pre-anthesis nitrogen metabolism meiosis stage rupturing stage |
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