| 环介导等温扩增技术在转基因作物成分检测中的应用 |
| |
| 引用本文： | 梁晋刚,杜再慧,黄春蒙,张飞燕,许文涛,张秀杰.环介导等温扩增技术在转基因作物成分检测中的应用[J].中国油料作物学报,2021,43(1):51. |
| |
| 作者姓名： | 梁晋刚 杜再慧 黄春蒙 张飞燕 许文涛 张秀杰 |
| |
| 作者单位： | 1. 农业农村部科技发展中心，北京，100176；
2. 中国检验检疫科学研究院，北京，100176；
3. 中国农业大学营养健康系，北京，100083 |
| |
| 基金项目： | 转基因生物新品种培育重大专项(2016ZX08012003)。 |
| |
| 摘 要： | 转基因作物成分检测为农业转基因生物安全管理提供了重要技术支撑。环介导等温扩增（Loop-mediat?
ed isothermal amplification，LAMP）技术操作简便、灵敏度高、特异性强，在转基因作物成分检测中得到了广泛应用。
本文针对近年来LAMP技术在转基因大豆、玉米和油菜成分检测中的应用情况进行了分析和总结，以期为LAMP技
术在转基因作物成分检测中的应用和标准化提供科学参考。
|
| 关 键 词： | 环介导等温扩增技术 转基因作物 成分检测 标准化 |
Loop-mediated isothermal amplification technology in the detection of genetically modified crops |
| |
| LIANG Jin-gang,DU Zai-hui,HUANG Chun-meng,ZHANG Fei-yan,XU Wen-tao,ZHANG Xiu-jie.Loop-mediated isothermal amplification technology in the detection of genetically modified crops[J].Chinese Journal of Oil Crop Sciences,2021,43(1):51. |
| |
| Authors: | LIANG Jin-gang DU Zai-hui HUANG Chun-meng ZHANG Fei-yan XU Wen-tao ZHANG Xiu-jie |
| |
| Institution: | 1. Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100176, China;
2. Chinese Academy of Inspection and Quarantine, Beijing 100176, China;
3. Department of Nutrition and Health,China Agricultural University, Beijing 100083, China |
| |
| Abstract: | The detection of genetically modified(GM)crops provides important technical support for the safety management of agricultural genetically modified organisms(GMOs).Loop-mediated isothermal amplification(LAMP)technology is easy to operate,efficient,sensitive,and specific.It has been widely used in the detection of GM crops.This article analyzes and summarizes the application of LAMP technology in the detection of GM soybean,maize and rapeseed in recent years,hoping to provide scientific references for further application and standardization of LAMP technology in the detection of GM crops. |
| |
| Keywords: | LAMP genetically modified crops components detection standardization |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国油料作物学报》浏览原始摘要信息 |
| 点击此处可从《中国油料作物学报》下载免费的PDF全文 |
|
