| 长发带芒草y型高分子量谷蛋白亚基的鉴定与分析 |
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| 引用本文： | 颜泽洪,代寿芬,刘登才,魏育明,郑有良.长发带芒草y型高分子量谷蛋白亚基的鉴定与分析[J].麦类作物学报,2005,25(6):44-46. |
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| 作者姓名： | 颜泽洪 代寿芬 刘登才 魏育明 郑有良 |
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| 作者单位： | 四川农业大学小麦研究所,都江堰,611830 |
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| 基金项目： | 高等学校全国优秀博士学位论文作者专项资金资助项目(200458)，国家自然科学基金资助项目(30370882)，四川省青年科技基金资助项目(04ZQ026-040) |
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| 摘 要： | 为了证实长发带芒草中的y型高分子量谷蛋白亚基的存在,利用SDS-PAGE分析了3份长发带芒草的高分子量谷蛋白亚基组成,发现其y亚基的迁移率均较普通小麦中迁移率最快的D y12亚基迁移率更快,应用PCR扩增、序列测定及基因编码区体外表达等方法研究了1份材料中的y亚基,确认了长发带芒草比普通小麦中迁移率最快的D y12亚基迁移率更快的T ay亚基的真实存在及表达。研究结果证实带芒草属具有与普通小麦中相类似的y型高分子量谷蛋白亚基。
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| 关 键 词： | 带芒草属 y型高分子量谷蛋白亚基 PCR扩增 序列分析 |
| 文章编号： | 1009-1041（2005）06-0044-03 |
| 收稿时间： | 2005-04-11 |
| 修稿时间： | 2005-06-30 |
Identifiaction of a y-type HMW-GS from Ta. crinitum Schreber Nevski |
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| YAN Ze-hong,DAI Shou-fen,LIU Deng-cai,WEI Yu-ming,ZHENG You-liang.Identifiaction of a y-type HMW-GS from Ta. crinitum Schreber Nevski[J].Journal of Triticeae Crops,2005,25(6):44-46. |
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| Authors: | YAN Ze-hong DAI Shou-fen LIU Deng-cai WEI Yu-ming ZHENG You-liang |
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| Institution: | Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China |
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| Abstract: | For a better understanding and utilization of y type HMW glutenins in Ta.crinitum,using SDS-PAGE the HMW glutenin compositions of 3 accession of Ta.crinitum were analysed,it was found that the electrophoresis mobility of the y type HMW glutenin subunits in Ta.crinitum were faster than that of Dy12 type HMW glutenin subunit,the fastest HMW glutenin subunit among y types of wheat.The novel y type HMW glutenin in Ta.crinitum,showing faster electrophoresis mobility than the smallest y type HMW glutenin subunit Dy12 in hexaploid wheat,was further confirmed by PCR amplification,gene sequencing and gene in vitro.The result revealed that Taeniatherum genus shares the similar y type HMW glutenin with those of hexaploid wheat. |
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| Keywords: | Taeniatherum y-type HMW glutenin PCR amplification Gene sequencing Gene expression |
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