| 盐生草HgNHX1基因在大麦株系中的功能验证 |
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| 引用本文： | 张 燕,李葆春,胡有良,汪军成,任盼荣,姚立蓉,马小乐,孟亚雄,杨柯,王化俊.盐生草HgNHX1基因在大麦株系中的功能验证[J].麦类作物学报,2018(8):929-934. |
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| 作者姓名： | 张 燕 李葆春 胡有良 汪军成 任盼荣 姚立蓉 马小乐 孟亚雄 杨柯 王化俊 |
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| 作者单位： | （1.甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室，甘肃兰州 730070；2.甘肃农业大学农学院，甘肃兰州 730070； 3.甘肃农业大学生命科学技术学院，甘肃兰州 730070） |
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| 基金项目： | 973计划前期研究专项（2014CB160313）；国家大麦青稞产业技术体系项目（CARS-05-04B-2）；国家自然科学基金地区项目（31460347） |
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| 摘 要： | 为了探讨盐和干旱环境胁迫对转盐生草液泡膜型Na+/H+逆向转运蛋白基因HgNHX1大麦幼苗生长及抗逆性生理指标的影响，以转HgNHX1基因大麦及野生型株系为材料，分析盐(150 mmol· L-1 NaCl)和自然干旱环境下转基因大麦幼苗的生长特性及生理指标的变化。结果表明，在盐胁迫条件下，随着胁迫时间的延长，转基因株系中HgNHX1 基因相对表达量逐渐上升；干旱胁迫条件下，转基因株系中HgNHX1 基因相对表达量呈先上升后下降的趋势，在处理3 d时达到最高。且在胁迫处理的三个不同时间点，转基因株系的相对含水量和游离脯胺酸含量均高于野生型株系，而相对电导率和丙二醛含量均低于野生型株系。这些结果说明，转基因株系中HgNHX1基因能够应答盐和干旱胁迫，具有提高大麦耐盐、抗旱性的功能。
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| 关 键 词： | 大麦 HgNHX1基因 盐胁迫 干旱胁迫 |
Functional Verification of HgNHX1 Gene Derived from Halogeton Glomeratus in Barley |
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| ZHANG Yan,LI Baochun,HU Youliang,WANG Juncheng,REN Panrong,YAO Lirong,MA Xiaole,MENG Yaxiong,YANG Ke,WANG Huajun.Functional Verification of HgNHX1 Gene Derived from Halogeton Glomeratus in Barley[J].Journal of Triticeae Crops,2018(8):929-934. |
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| Authors: | ZHANG Yan LI Baochun HU Youliang WANG Juncheng REN Panrong YAO Lirong MA Xiaole MENG Yaxiong YANG Ke WANG Huajun |
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| Abstract: | In order to study the effects of salt and drought stress on the growth and stress resistance physiological indices of barley seedlings transformed with a Halogeton glomeratus vacuolar Na+/H+ antiporter gene HgNHX1, the HgNHX1 transgenic barley and wild type strains were used as materials, to analyse the changes of growth and physiological characteristics under salt (150 mmol·L-1 NaCl) stress and the natural arid environment. The results showed that under salt stress, the relative expression of HgNHX1 gene in transgenic lines increased gradually with the prolonging of stress time. With the extension of drought stress time, the relative expression of HgNHX1 gene in transgenic lines gradually increased, and reached the highest level at 3 days. At three different time points of stress treatment, the RWC and L-Pro of the transgenic lines were higher than those of the wild type, but REC and MDA were lower than those of the wild type. These results showed that HgNHX1 gene in transgenic lines could respond to salt and drought stress, and this gene could improve the salt tolerance and drought resistance of barley. |
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| Keywords: | Barley HgNHX1gene Salinity stress Drought stress |
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