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| 记录小麦SSR带型的快捷方法 | |
| 引用本文： | 王立新,赵昌平,邱军,李宏博,葛玲玲,孙辉,姚骥.记录小麦SSR带型的快捷方法[J].麦类作物学报,2006,26(4):164-168. |
| 作者姓名： | 王立新 赵昌平 邱军 李宏博 葛玲玲 孙辉 姚骥 |
| 作者单位： | 1. 北京杂交小麦工程技术研究中心,北京,100089 2. 全国农业技术推广服务中心,北京,100026 3. 华中农业大学生命科学技术学院,湖北,武汉,430070 |
| 基金项目： | 北京市农业育种基础研究创新平台项目;农业部全国农业技术推广服务中心项目 |
| 摘 要： | 针对小麦SSR带型识别和记录中因带纹繁多和分布复杂而造成带纹判读和记录困难的特点，提出了根据每对SSR引物生成带型间的差别直接对带型进行编号，以有效简化小麦SSR带型的记录方法。该方法避免了由于错误判断引起的实验误差，并可利用每个品种的带型编号，建立品种的DNA指纹以及进行品种基因型比对。 |
| 关 键 词： | 小麦 SSR带型 识别 |
| 文章编号： | 1004-1389（2006）04-0164-05 |
| 收稿时间： | 2005-09-27 |
| 修稿时间： | 2005-12-22 |
A New Scoring Method of SSR Patterns for Wheat |
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| WANG Li-xin,ZHAO Chang-ping,QIU Jun,LI Hong-bo,Ge Ling-ling,SUN Hui,YAO Ji.A New Scoring Method of SSR Patterns for Wheat[J].Journal of Triticeae Crops,2006,26(4):164-168. | |
| Authors: | WANG Li-xin ZHAO Chang-ping QIU Jun LI Hong-bo Ge Ling-ling SUN Hui YAO Ji |
| Institution: | 1. Beijing Engineering and Technique Research Center of Hybrid Wheat, Beijing 100089, China; 2. National Agriculture Technique Development Service, Beijing 100026,China; 3. College of Life Science and technology, Huazhong Agricultural University, Wuhan, Hubei 430070,China |
| Abstract: | SSR markers are widely used to establish the crop DNA fingerprint. However, the SSR patterns of wheat have more bands and are more complex than other crops. Therefore, it is difficult to score each band with number 1 and 0 when a piece of gel appears with 50-60 lanes. Here, we score each SSR pattern with two digits instead of scoring each band with many pairs of land 0. By arranging all of pattern numbers, we can build the fingerprint code for every wheat accession, and thus, each fingerprint code could be easily converted into computer codes. |
| Keywords: | Wheat SSR patterns Labeling |
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