| 一个水稻类树稻突变体(leafy head 3)基因的精细定位 |
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| 引用本文： | 吴立文,姜华,胡江,胡兴明,高振宇,曾大力,朱观林,董国军,钱前,郭龙彪.一个水稻类树稻突变体(leafy head 3)基因的精细定位[J].中国水稻科学,2009,23(6):583-588. |
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| 作者姓名： | 吴立文 姜华 胡江 胡兴明 高振宇 曾大力 朱观林 董国军 钱前 郭龙彪 |
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| 作者单位： | 中国水稻研究所,水稻生物学国家重点实验室,浙江,杭州,310006 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 从常规粳稻常优94后代中筛选到一份自然突变的抽穗期延迟的类树稻突变体lhd3（leafy head 3）。在短日照条件下，与野生型比较，lhd3突变体在生长后期，上部节间会继续长出叶片（一般为3片）和高位分蘖，类似于树的侧枝生长，抽穗期延迟，但基部分蘖数不受影响。经典遗传分析表明，lhd3与籼稻南京6号的F2群体中，正常植株与类树稻植株的分离比符合3∶1，说明此性状受单隐性基因控制。利用该群体进行图位克隆，将LHD3基因定位在水稻第1染色体短臂的两个新发展的STS标记wpla3和wpla25之间。再利用5个新发展的STS和CAPS标记，最终将该基因精细定位在WX6和CAPS1两个标记之间，物理距离约为60 kb。通过水稻基因组注释系统共预测到10个开放阅读框（ORF）。对该基因的进一步克隆将有助于阐明水稻生育期和叶原基发育调控机理。
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| 关 键 词： | 水稻 突变体 抽穗期 基因 精细定位 |
| 收稿时间： | 1900-01-01; |
Genetic Analysis and Fine-Mapping of a Leafy Head 3 Mutant in Rice |
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| WU Li-wen,JIANG Hua,HU Jiang,HU Xing-ming,GAO Zhen-yu,ZENG Da-li,ZHU Guan-lin,DONG Guo-jun,QIAN Qian,GUO Long-biao.Genetic Analysis and Fine-Mapping of a Leafy Head 3 Mutant in Rice[J].Chinese Journal of Rice Science,2009,23(6):583-588. |
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| Authors: | WU Li-wen JIANG Hua HU Jiang HU Xing-ming GAO Zhen-yu ZENG Da-li ZHU Guan-lin DONG Guo-jun QIAN Qian GUO Long-biao |
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| Institution: | State Key Laboratory of Rice Biology， China National Rice Research Institute， Hangzhou 310006, China; *Corresponding author, E-mail: guolongb@mail.hz.zj.cn |
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| Abstract: | A natural rice mutant lhd3(leafy head 3) with delayed heading date was isolated from progenies of a japonica cultivar, Changyou 94. Under short-day conditions, outgrowth of new leaves (generally 3 leaves) and tillering on upper nodes continues at the late stage of vegetative growth in the lhdd3 mutant, which is similar to tree branching. Also it holds the delay in heading date. Classical genetic analysis on F_2 population of lhd3/Nanjing 6 indicated that the lhd3 phenotype was controlled by a single recessive gene. LHD3 gene was mapped with the F_2 population between two newly-developed STS markers, wpla3 and wpla25. on the short arm of rice chromosome 1. Fine mapping delimited it to a 60 kb physical distance between a STS marker and a CAPS marker, WX6 and CAPS1, where 10 ORFs were predicted by Rice Genome Annotation (http://rice. plantbiology. msu. edu/index. shtml). Further cloning of LHD3 will contribute to the clarification of regulation mechanism for growth period and leaf-primordium development in rice. |
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| Keywords: | rice mutant heading date gene fine mapping |
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