| 一个水稻卷叶基因rl(t)的精细定位 |
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| 引用本文： | 潘存红,李磊,陈宗祥,薛芗,张亚芳,左示敏,戴正元,潘学彪,马玉银.一个水稻卷叶基因rl(t)的精细定位[J].中国水稻科学,2011,25(5):455-460. |
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| 作者姓名： | 潘存红 李磊 陈宗祥 薛芗 张亚芳 左示敏 戴正元 潘学彪 马玉银 |
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| 作者单位： | 扬州大学江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室;江苏里下河地区农业科学研究所/国家水稻改良中心南京分中心;扬州职业大学生化工程系; |
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| 基金项目： | 江苏省自然科学基金资助项目(BK2008223,BK2009696); 江苏高校省级重点实验室开放课题资助项目(K09004) |
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| 摘 要： | 叶片适度卷曲是水稻理想株型塑造的重要组成部分。卷叶基因rl(t)在杂合状态下可使叶片适度卷曲，增加水稻产量，具有较高的育种利用价值。以卷叶珍汕97B\携带rl(t)基因\]与平展叶品种奇妙香（轮回亲本）杂交并回交的BC5F3和BC5F4为定位群体，在前人关于rl(t)的定位区间内设计了15对多态性分子标记，将rl(t)精细定位于第2染色体上分子标记P95053和P113.6之间的11 kb范围内，遗传间距约0.04 cM。区间内只包含1个释义基因，预测编码GL2类同源异型结构域，属于同源异型盒家族中的HD GL2类（也称为HD ZIP Ⅳ）转录因子。卷叶基因rl(t)的精细定位为克隆目标基因和研究其调控机理奠定了基础。
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| 关 键 词： | 水稻 卷叶基因 精细定位 |
| 收稿时间： | 2010-10-26; |
Fine Mapping of a Rolled Leaf Gene rl_(t) in Rice |
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| PAN Cun-hong,LI Lei,CHEN Zong-xiang,XUE Xiang,ZHANG Ya-fang,ZUO Shi-min,DAI Zheng-yuan,PAN Xue-biao,MA Yu-yin.Fine Mapping of a Rolled Leaf Gene rl_(t) in Rice[J].Chinese Journal of Rice Science,2011,25(5):455-460. |
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| Authors: | PAN Cun-hong LI Lei CHEN Zong-xiang XUE Xiang ZHANG Ya-fang ZUO Shi-min DAI Zheng-yuan PAN Xue-biao MA Yu-yin |
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| Institution: | PAN Cun-hong1,2,LI Lei1,CHEN Zong-xiang1,XUE Xiang1,ZHANG Ya-fang1,ZUO Shi-min1,DAI Zheng-yuan2,PAN Xue-biao1MA Yu-yin1,3,(1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of Ministry of Education,Yangzhou University,Yangzhou 225009,China,2 Lixiahe Agricultural Research Institute of Jiangsu Province/Nanjing Subcenter of National Rice Improvement Center,Yangzhou 225007,3 Biochemical Engineering Department,Yangzhou Polytechnic College,Yan... |
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| Abstract: | Moderately rolled leaf is one of the most important morphological characters in ideal plant architecture breeding in rice.The heterozygous rl(t) gene could produce moderately rolled leaf and increase rice yield,presenting a high breeding potential.By using BC5F3 and BC5F4 populations derived from a rolled leaf Zhenshan 97B(carrying rl(t) gene) and a flat-leaf variety Qimiaoxiang(recurrent parent),and a total of 15 polymorphic markers,the rl(t) gene was ultimately mapped into a region of 11 kb flanked by the... |
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| Keywords: | rice rolled leaf gene fine mapping |
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