| 水稻淡绿叶突变体HM133的遗传分析与基因定位 |
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| 引用本文： | 施勇烽,#,贺彦,#,郭丹,吕向光,黄奇娜,吴建利.水稻淡绿叶突变体HM133的遗传分析与基因定位[J].中国水稻科学,2016,30(6):603-610. |
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| 作者姓名： | 施勇烽 # 贺彦 # 郭丹 吕向光 黄奇娜 吴建利 |
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| 作者单位： | 1.中国水稻研究所 水稻生物学国家重点实验室/国家水稻改良中心， 杭州 310006；;2.中国农业科学院 作物科学研究所， 北京 100081 |
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| 摘 要： | EMS诱导籼稻品种IR64获得淡绿叶突变体HM133。与野生型IR64相比,HM133播种后的第6周和第15周的光合色素含量以及抽穗期的净光合速率显著降低,气孔导度则明显上升;此外,突变体株高、每穗实粒数和结实率等农艺性状也较野生型显著下降。叶绿体超微结构分析表明,分蘖期HM133类囊体基粒片层形状不规则,堆叠凌乱、排列疏松。遗传分析表明HM133淡绿叶性状受单隐性核基因控制。通过分子标记将该基因定位于第3染色体长臂RM143和RM3684之间。该区间内包含编码镁螯合酶D亚基的基因OsCHLD。序列分析表明HM133中该基因第10外显子上有一个从G突变为A的单碱基变异,导致编码的氨基酸由精氨酸变成谷氨酸,推测OsCHLD基因即为控制HM133淡绿叶表型的候选基因。
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| 关 键 词： | 水稻 淡绿叶 基因定位 镁螯合酶 光合作用 |
| 收稿时间： | 2016-03-02 |
Genetic Analysis and Gene Mapping of a Pale Green Leaf Mutant HM133 in Rice |
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| SHI Yong feng,#,HE Yan,#,GUO Dan,LV Xiang guang,HUANG Qi na,WU Jian li.Genetic Analysis and Gene Mapping of a Pale Green Leaf Mutant HM133 in Rice[J].Chinese Journal of Rice Science,2016,30(6):603-610. |
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| Authors: | SHI Yong feng # HE Yan # GUO Dan LV Xiang guang HUANG Qi na WU Jian li |
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| Institution: | 1.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; |
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| Abstract: | The pale green leaf mutant HM133 was identified from an EMS induced IR64 mutant bank. The contents of photosynthetic pigments including chlorophyll and carotenoid of HM133 were reduced significantly at 6 weeks and 15 weeks after sowing when compared with IR64.The net photosynthetic rate of HM133 was considerably lower than that of the wild type IR64 at heading stage while the stomatal conductance was apparently increased. The agronomic traits including plant height, number of filled grain per panicle and seed setting rate decreased significantly in the mutant compared with the wild type. In addition, the mutant exhibited a less number of grana, irregular arrangement of thylakoid layer in the chloroplast at the tillering stage. Genetic and mapping analysis showed that the pale green phenotype was controlled by a single recessive gene located in the long arm of chromosome 3 between SSR markers RM143 and RM3684. The interval contains an ORF OsChlD encoding magnesium chelatase D subunit. Sequence analysis revealed that the mutant allele carried a nucleotide substitution from G to A in the tenth exon of OsChlD, which led to the substitution of glutamic acid for arginine acid. Therefore, it is deduced that OsChlD is the candidate gene controlling the pale green leaf phenotype of HM133. |
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| Keywords: | Oryza sativa pale green leaf gene mapping magnesium chelatase photosynthesis |
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