| 核酸适配体传感器在常见食源性致病菌检测中的应用及发展 |
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| 引用本文： | 王晓颖,黄 键,王晓惠,孙雪婷,高雪梅,施锦辉.核酸适配体传感器在常见食源性致病菌检测中的应用及发展[J].农业工程,2024,14(2). |
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| 作者姓名： | 王晓颖 黄 键 王晓惠 孙雪婷 高雪梅 施锦辉 |
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| 作者单位： | 南通海关综合技术中心,南通海关综合技术中心,南通市如皋生态环境监测站,南通海关综合技术中心,南通海关综合技术中心,南通海关综合技术中心 |
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| 基金项目： | 南京海关科研项目(2022KJ27) |
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| 摘 要： | 食源性致病菌是食源性疾病的重要诱因,早期的筛查技术已不能满足对食源性致病菌的快速、准确检测要求。核酸适配体因其小尺寸、易修饰、亲和性高、热稳定等优点而被广泛应用,研究者们运用核酸适配体传感器开发了针对食源性致病菌的众多检测方法,用于快速筛查食源性致病菌。该文介绍了食源性致病菌的常用检测方法,总结归纳了核酸适配体的筛选程序,列举了核酸适配体传感器在食源性致病菌检测中的应用,分析了其在应对样品的快速高效检测要求时的发展趋势,以求为食源性致病菌的检测方法改进提供更多思路。
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| 关 键 词： | 食源性致病菌 快速检测 核酸适配体 适配体筛选 核酸适配体传感器 |
| 收稿时间： | 2023/7/2 0:00:00 |
| 修稿时间： | 2023/10/23 0:00:00 |
Application and Development of Aptamer Sensor in the Detection of Common Foodborne Pathogens |
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| Wang Xiaoying,Huang Jian,Wang Xiaohui,Sun Xueting,Gao Xuemei and Shi Jinhui.Application and Development of Aptamer Sensor in the Detection of Common Foodborne Pathogens[J].Agricultural Engineering,2024,14(2). |
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| Authors: | Wang Xiaoying Huang Jian Wang Xiaohui Sun Xueting Gao Xuemei and Shi Jinhui |
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| Institution: | Nantong Customs comprehensive technology Center,Nantong Customs comprehensive technology Center,Nantong Rugao Ecological Environment Monitoring Station,Rugao,Nantong Customs comprehensive technology Center,Nantong Customs comprehensive technology Center,Nantong Customs comprehensive technology Center |
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| Abstract: | Foodborne pathogens are an important cause of foodborne diseases, and early screening technology can not meet the requirements of rapid and accurate detection of foodborne pathogens. Aptamers are widely used because of their advantages such as small size, easy modification, high affinity and heat stability. Researchers have developed many detection methods for foodborne pathogens by using aptamer sensors for rapid screening of foodborne pathogens. This article introduces the common detection methods of foodborne pathogens, summarizes the screening procedures of nucleic acid aptamer, lists the application of nucleic acid aptamer sensor in the detection of foodborne pathogens, and analyzes its development trend in response to the rapid and efficient detection requirements of samples, in order to provide more ideas for the improvement of detection methods of foodborne pathogens. |
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| Keywords: | foodborne pathogens rapid detection nucleic acid aptamer aptamer screening nucleic acid aptamer sensor |
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