| 笋用竹组培快繁及其种苗产业化 |
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| 引用本文： | 陈国华,陈冬怡,王强,胡群英,黄春华,黄芳,叶秀粦.笋用竹组培快繁及其种苗产业化[J].农业工程,2018,8(11):115-119. |
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| 作者姓名： | 陈国华 陈冬怡 王强 胡群英 黄春华 黄芳 叶秀粦 |
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| 作者单位： | 中国科学院华南植物园，广东省应用植物学重点实验室，广东 广州510650 |
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| 摘 要： | 以苏麻竹等笋用竹的顶芽、侧芽和根等为外植体，通过诱导培养形成脱毒不定芽，通过组培快繁技术，培养出完整的无毒植株。试验结果表明:外植体灭菌处理成功率达78%，诱导不定芽的最适培养基为MS+6-BA3.0+IBA0.2+NAA0.1，不定芽继代增殖的最适培养基为MS+6-BA2.0+IBA0.2+NAA0.1，增殖系数为2.32，生根率达91%，移栽成活率达93%，为笋用竹种苗提供了一种新型快速的繁殖方式。
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| 关 键 词： | 笋用竹 快繁 种苗 |
Tissue Culture and Rapid Propagation of Shoot-producing Bamboo and Seeding Industriulization |
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| CHEN Guohu,CHEN Dongyi,WANG Qiang,HU Qunying,HUANG Chunhu,HUANG Fang and YE Xiulin.Tissue Culture and Rapid Propagation of Shoot-producing Bamboo and Seeding Industriulization[J].Agricultural Engineering,2018,8(11):115-119. |
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| Authors: | CHEN Guohu CHEN Dongyi WANG Qiang HU Qunying HUANG Chunhu HUANG Fang and YE Xiulin |
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| Abstract: | Terminal buds，lateral buds and roots of some kinds of shoot-producing bamboo like Dendrocalamus latiflorus Munro were taken as explants，adventitious buds were successfully induced by tissue culture，after rapid propagating，complete and virus-free plants were obtained.Results showed that explants sterilization success rate was as high as 78%.Optimal medium for adventitious buds induction was MS+6-BA3.0+IBA0.2+NAA0.1，optimizing medium for subculture multiplication was MS+6-BA2.0+IBA0.2+NAA0.1，and subculture multiplication factor was up to 2.32，rooting rate was 91% and transplanting survival rate was 93%，is could provide an effect way for rapid propagation of virus-free shoot-producing bamboo seedlings. |
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