| 柑橘脱毒快繁及微芽嫁接技术试验研究 |
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| 引用本文： | 陈国华,陈冬怡,马文卿,陈新桃,黄春华,胡群英,黄芳,叶秀粦.柑橘脱毒快繁及微芽嫁接技术试验研究[J].农业工程,2017,7(2):129-132. |
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| 作者姓名： | 陈国华 陈冬怡 马文卿 陈新桃 黄春华 胡群英 黄芳 叶秀粦 |
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| 作者单位： | 广东省应用植物学重点实验室，中国科学院华南植物园，广州510650 |
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| 基金项目： | 广东省科技计划项目“柑橘茎尖脱毒快繁与试管内微芽嫁接技术的研究与示范”(2014A020208080) |
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| 摘 要： | 以红江橙、沙糖桔的顶芽、侧芽和茎段为外植体，通过诱导培养形成脱毒不定芽接穗；通过微芽嫁接于砧木实生苗，培养成完整的无毒植株。试验结果表明:外植体灭菌处理成功率达86%，诱导不定芽的最适培养基为MT+6-BA 3.0 mg/L+IBA 0.1 mg/L+KT 0.1 mg/L；不定芽继代增殖的最适培养基为MT+6-BA 2.0 mg/L+IBA 0.1 mg/L，增殖系数为2.3；砧木种子播种成苗率达90%；微芽嫁接愈合成活率平均达87%。该研究为柑橘脱毒种苗提供了一种新型快速的繁殖方式。
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| 关 键 词： | 柑橘 脱毒 快繁技术 微芽嫁接 |
Test and Research on Virus-free Rapid Propagation Technology and Micro Bud Grafting Technology of Citrus |
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| Chen Guohu,Chen Dongyi,Ma Wenqing,Chen Xintao,Huang Chunhu,Hu Qunying,Huang Fang and Ye Xiulin.Test and Research on Virus-free Rapid Propagation Technology and Micro Bud Grafting Technology of Citrus[J].Agricultural Engineering,2017,7(2):129-132. |
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| Authors: | Chen Guohu Chen Dongyi Ma Wenqing Chen Xintao Huang Chunhu Hu Qunying Huang Fang and Ye Xiulin |
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| Abstract: | Using Citrus sinensis Osbeck cv.Hongjiang and Citrus Shatangju，virus-free adventitious buds were formed by inducing culture.Complete non-toxic plants were cultured by grafting seedling to rootstock seedling.Test results showed that success rate of explant sterilization was 86%，optimum medium of induction of adventitious buds was MT+6-BA 3.0 mg/L+ IBA 0.1 mg/L+KT 0.1 mg/L.Optimum medium of adventitious bud subculture was MT+6-BA 2.0 mg/L+IBA 0.1 mg/L and proliferation coefficient was 2.3.Seedling rate of stock seed reached 90%.Average survival rate of micrograft reached 87%.This study provided a new way of rapid propagation for Citrus virus-free seedling. |
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