| 黑尾近红鲌精子低温保存方法研究与应用 |
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| 引用本文： | 王贵英,张涵,李清,祝东梅,陈见,李佩,孙艳红.黑尾近红鲌精子低温保存方法研究与应用[J].淡水渔业,2016(6). |
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| 作者姓名： | 王贵英 张涵 李清 祝东梅 陈见 李佩 孙艳红 |
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| 作者单位： | 1. 武汉市农业科学技术研究院水产科学研究所，武汉 430207; 武汉先锋水产科技有限公司，武汉 430207;2. 武汉先锋水产科技有限公司，武汉 430207; 湖北省潜江市水产技术推广中心，湖北潜江 433199 |
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| 基金项目： | 国家科技计划项目(2015BAD25B01-5)，武汉市黄鹤英才计划项目(武人才办[2014]8号;序号19)，武汉市科技计划项目(20032003035、2013020705070350-15、2014020202010137) |
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| 摘 要： | 在4℃条件下,以精子活力为指标,研究了不同浓度的Na~+、K~+、Ca~(2+)、Mg~(2+)、葡萄糖、氨基酸等组成的5种(A、B、C、D、E)精子保存液及其适量添加青霉素对黑尾近红鲌(Ancherythroculter nigrocauda)精子活力的影响。结果显示:1)温度为4℃时,精子活力达80%的保存液A、B、C、D、E的保存时间分别为48、24、60、48、48 h,保存液C的保存时间明显高于其它各组;2)在各保存液中分别添加浓度为2.0×104IU/m L的青霉素,精子活力达80%的保存液A、B、C、D、E的保存时间分别为60、36、156、48、144 h,保存时间延长了12~96 h,保存液C和E的延长时间最长,均为96 h;3)人工授精试验证明,经保存的黑尾近红鲌精子能正常用于人工繁殖,受精率达(90.6±0.8)%~(91.8±0.9)%,与对照组精子受精率(92.4±0.8)%无显著差异。添加青霉素,保存液C的保存效果最好,其次是保存液E。
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| 关 键 词： | 黑尾近红鲌(Ancherythroculter nigrocauda) 精子 保存液 青霉素 保存效果 |
Preservation of sperm from Ancherythroculter nigrocauda at low temperature and its application |
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| Abstract: | Under the condition of low temperature (4oC), we studied the effects of 5 kinds of sperm preservation solutions (A, B, C, D, E) containing different concentration of Na+, K+, Ca2+, Mg2+, amino acid, glucose and adding moderate amount of penicillin on the sperm motility in Ancherythroculter nigrocauda, to explored the best preservation con-dition of the sperm vitality as indicators. The results showed that: 1) Under the temperature of 4 ℃, the preservative so-lution of A, B, C, D and E could keep 80% of spermatozoa having fast motility within 48, 24, 60, 48, 48 h respec-tively, and the preservation time of C solution was obviously higher than that of other groups; 2) With the addition of 2. 0 × 104 IU/mL penicillin, A, B, C, D and E could keep 80% of spermatozoa having fast motility within 60, 36, 156, 48, 144 h respectively, and the preservation time were prolonged for 12 h ~ 96 h. C and E solutions were extended to the longest time both with 96 h; 3 ) The experiment of artificial insemination proved that the preserved sperm of A. nigrocauda could be normally used for artificial breeding, and the fertilization rate (90. 6 ± 0. 8)% ~(91. 8 ± 0. 9)%had no significant difference (p>0. 05) compared with control group fresh sperm (92. 4 ± 0. 8%). The results indicated that among the preservation solutions with addition of 2. 0 × 104 IU/mL penicillin, the C solution had the best preservation effect, followed by E, which could be applied in the factory production. |
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| Keywords: | Ancherythroculter nigrocauda sperm preservation solution penicillin preservation effect |
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