| 塔里木裂腹鱼群体遗传多样性的AFLP分析 |
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| 引用本文： | 杨天燕,郭焱,孟玮,马燕武,陈朋.塔里木裂腹鱼群体遗传多样性的AFLP分析[J].淡水渔业,2012(4):83-87. |
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| 作者姓名： | 杨天燕 郭焱 孟玮 马燕武 陈朋 |
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| 作者单位： | 新疆维吾尔自治区水产科学研究所 |
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| 基金项目： | 新疆维吾尔自治区自然科学基金项目(2009211A30);“国家科技基础条件平台-水产种质资源平台”项目;国家自然科学基金项目(31060356) |
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| 摘 要： | 采用AFLP技术对喀拉喀什河、塔什库尔干河、阿克苏河多浪渠首、木扎提河4个群体共80尾塔里木裂腹鱼(Schizothorax biddulphi Günther)个体进行了遗传多样性分析。6对选择性扩增引物共扩增得到212个位点,其中多态性位点141个,多态位点比例为66.51%。4个群体的Shannon多样性指数(I)为0.316 9-0.544 3,Nei’s遗传多样性指数(H)为0.213 9-0.376 2,群体间的遗传距离为0.078 1-0.250 2。塔什库尔干河群体的多态位点比例、Shannon多样性指数和Nei’s遗传多样性指数均高于其它三个群体。AMOVA分析结果显示,群体总遗传变异85.17%来自群体内差异,而14.83%来自群体间差异,表明塔里木裂腹鱼遗传变异主要来源于群体内个体间,但群体间已存在一定程度的遗传分化。用UPGMA方法构建的群体系统进化树显示,多浪渠首群体和木扎提河群体首先聚类,然后依次与塔什库尔干河群体、喀拉喀什河群体聚类,这一方面与地理位置有关,另一方面与河流的自然环境有关。
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| 关 键 词： | 塔里木裂腹鱼(Schizothorax biddulphi) AFLP分析 遗传多样性 |
AFLP analysis of genetic diversity of Schizothorax biddulphipopulations |
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| YANG Tian-yan,GUO Yan,MENG Wei,MA Yan-wu,CHEN Peng.AFLP analysis of genetic diversity of Schizothorax biddulphipopulations[J].Freshwater Fisheries,2012(4):83-87. |
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| Authors: | YANG Tian-yan GUO Yan MENG Wei MA Yan-wu CHEN Peng |
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| Institution: | (Xinjiang Fisheries Research Institute,Urumqi 830000) |
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| Abstract: | The genetic diversity of 80 Schizothorax biddulphi individuals collected from Kalakashi River(K),Tashikuergan River(T),Duolang canal head of Akesu River(D) and Muzhati River(M) were analyzed by AFLP markers.A total of 212 loci were acquired by using 6 pairs of selective amplification primers,141 of which were polymorphic,and the percentage of polymorphic loci was 66.51%.The Shannon diversity indices ranged from 0.316 9 to 0.544 3,the Nei’s gene diversity indices varied from 0.213 9 to 0.376 2,and the genetic distances among different populations were from 0.078 1 to 0.250 2.The percentage of polymorphic loci,Shannon diversity index,and Nei’ s gene diversity index in Tashikuergan River population were higher than the corresponding values in other populations.The AMOVA results showed that variance within populations was 85.17% and among populations was 14.83%.It suggested that the genetic variation occurred mainly within populations,but a certain degree of genetic differentiation existed among populations.The phylogeny tree constructed by UPGMA method revealed that the Duolang canal head population and Muzhati River population were first clustered together,and then gathered with Tashikuergan population and Kalakashi River population respectively.The geographical locations and river’ s natural environment might be the main factors affecting the genetic diversity pattern of S.biddulphi. |
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| Keywords: | Schizothorax biddulphi AFLP analysis genetic diversity |
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