| 尼罗罗非鱼淀粉酶性质的初步研究 |
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| 引用本文： | 谢进金,蒋娜红,洪绿萍,蔡炳炎.尼罗罗非鱼淀粉酶性质的初步研究[J].淡水渔业,2007,37(2):34-37. |
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| 作者姓名： | 谢进金 蒋娜红 洪绿萍 蔡炳炎 |
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| 作者单位： | 泉州师范学院生物学系,生物化学与分子生物学研究所,福建泉州,362000 |
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| 基金项目： | 泉州师范学院校科研和教改项目，大学生科研基金 |
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| 摘 要： | 研究了尼罗罗非鱼(Oreochromis niloticus)肝脏淀粉酶的基本性质及金属离子对该鱼淀粉酶活性的影响。结果表明:罗非鱼淀粉酶活力的最适pH是6.5,最适底物浓度是2%。研究金属离子对该鱼淀粉酶活力影响,一价金属离子K+、Li+、Na+对酶活力影响较小;二价金属离子Cu2+对酶活力具有抑制作用,Zn2+对酶活力无明显影响;三价金属离子Al3+对酶活力具有抑制作用,但效果不是很强烈,Fe3+对酶活力具有明显的激活作用;碱土金属Mg2+、Ca2+对淀粉酶活性有激活作用,而Ba2+对酶具有抑制作用;重金属离子Cd2+、Pb2+有明显的抑制作用。
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| 关 键 词： | 尼罗罗非鱼(Oreochromis niloticus) 淀粉酶活力 金属离子 |
| 文章编号： | 1000-6907-（2007）02-0034-04 |
| 修稿时间： | 2006-06-18 |
Primary Study on Properties of Amylase from Nile Tilapia (Oreochromis niloticus) |
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| XIE Jin-jin,JIANG Na-hong,HONG Lv-ping,CAI Bing-yan.Primary Study on Properties of Amylase from Nile Tilapia (Oreochromis niloticus)[J].Freshwater Fisheries,2007,37(2):34-37. |
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| Authors: | XIE Jin-jin JIANG Na-hong HONG Lv-ping CAI Bing-yan |
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| Institution: | Biology Department,Institute of Biochemistry and Molecule Biology, Quanzhou Normal University, Quanzhou,Fujian 362000 |
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| Abstract: | Properties of amylase from Nile tilapia(Oreochromis niloticus) was studies in this paper.The result showed that:the optimum pH for amylase was 6.5,the optimum substrate concentration was 2%.The effects of some metal ions on the amylase activity were studied.And the positive univalent metal ions like K+、Li+ and Na+ had little influence on the enzyme activity.The positive bivalent metal ions inhibit the enzyme and the order of the inhibit intensity was as follows:Cu2+>Zn2+.And positive bivalent alkaline earth metalions(Mg2+,Ca2+ and Ba2+)affected the enzyme and the order of the activation intensity was as follows:Mg2+>Ca2+,but Ba2+ inhibit the enzyme activity.The positive trivalent metal ions Al3+ had the inhibitory activity,but the effect was unobvious,and Fe3+ had the activation effect.And heavy metal ions Cd2+、Pb2+ inhibited the enzyme strongly. |
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| Keywords: | Nile tilapia(Oreochromis niloticus) amylase activity metal ions |
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