| 黄颡鱼吻端细胞培养研究 |
| |
| 引用本文： | 孔祥婷,沈颂东,姜德勋,姜红霞,徐健荣,张君,韩晓磊,许璞.黄颡鱼吻端细胞培养研究[J].淡水渔业,2009,39(2). |
| |
| 作者姓名： | 孔祥婷 沈颂东 姜德勋 姜红霞 徐健荣 张君 韩晓磊 许璞 |
| |
| 作者单位： | 1. 苏州大学细胞生物学系,江苏苏州,215123
2. 常熟理工学院生物与食品工程系,江苏常熟,215500 |
| |
| 摘 要： | 采用M199培养基对黄颡鱼(Pelteobagrus fulvidraco)吻端组织进行体外培养研究,继代培养细胞传至第8代。实验结果显示:吻端组织接种培养3~5 h,细胞迁移伸展,6 d后生长细胞集落汇合,每4~6 d递次进行继代培养,培养生长的细胞呈纤维样。比较继代细胞在不同温度(15、27、37℃)和血清浓度(0、10%、20%)条件下的生长,27℃、10%血清的培养条件最适宜吻端细胞的增殖。通过染色体组型分析,证明离体吻端细胞为正常的二倍体细胞。研究结果表明,黄颡鱼离体吻端细胞对温度等培养条件有很强的适应性,培养细胞增殖迅速,具有建立连续细胞系的潜力。
|
| 关 键 词： | 黄颡鱼(Pelteobagrus fulvidraco) 吻端细胞 继代培养 染色体 |
A preliminary study on snout tissue culture from Pelteobagrus fulvidraco |
| |
| KONG Xiang-ting,SHEN Song-dong,JIANG De-xun,JIANG Hong-xia,XU Jian-rong,ZHANG Jun,HAN Xiao-lei,XU Pu.A preliminary study on snout tissue culture from Pelteobagrus fulvidraco[J].Freshwater Fisheries,2009,39(2). |
| |
| Authors: | KONG Xiang-ting SHEN Song-dong JIANG De-xun JIANG Hong-xia XU Jian-rong ZHANG Jun HAN Xiao-lei XU Pu |
| |
| Institution: | KONG Xiang-ting1,SHEN Song-dong1,JIANG De-xun2,JIANG Hong-xia2,XU Jian-rong2,ZHANG Jun2,HAN Xiao-lei2,XU Pu2(1.Department of Cell Biology,Soochow University,Suzhou,Jiangsu 215123,2.Department of Biology and Food Engineering,Changshu Institute of Technology,Changshu,Jiangsu 215500) |
| |
| Abstract: | The cells of snout tissues derived from Pelteobagrus fulvidraco were studied in vitro with the medium 199.The snout cells have been subcultured to the eighth generation.The snout cells migrated from the tissues after cultured in vitro for 3~5 h.After that,cells could be done once every 4or 6 days.The cells were fiber-like cells in primary culture and subculture.When cultured under different temperature(15,27,37 ℃) and serum concentration(0,10%,20%),cells were most quickly multiplied under 27 ℃ and 10% serum concentration.Chromosome research demonstrated that the snout cells in vitro were normal diploid cells(2n=52).The results showed that the snout tissue of P.fulvidraco reproduced rapidly and cultivated cells could adapt broad temperature in vitro.Therefore,they had the potential ability to become the tissue source of establishing continuous cell line. |
| |
| Keywords: | Pelteobagrus fulvidraco snout tissue subculture chromosome |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
