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Genetic analysis of Aequipecten opercularis and Mimachlamys varia (Bivalvia: Pectinidae) in several Atlantic and Mediterranean localities, revealed by mitochondrial PCR-RFLPs: a preliminary study
Authors:Mercedes Fernandez-Moreno  Alberto Arias-Perez  Ruth Freire  & Josefina Méndez
Institution:Department of Cell and Molecular Biology, Faculty of Sciences, University of A Coruña, A Coruña, Galicia, Spain
Abstract:Aequipecten opercularis (Queen scallop) and Mimachlamys (Chlamys) varia (Black scallop) are important natural resources occurring in Atlantic and Mediterranean coasts. To develop an optimal sustainable exploitation plan, it is important to study the genetic structure of the different populations. In this study, we used polymerase chain reaction‐restriction fragment length polymorphisms for the determination of the genetic variation and population structure of these two species in different localities. Ten composite haplotypes were generated for A. opercularis and 15 haplotypes for M. varia. Of these, six and four were unique respectively. The analysis of the distribution of the different haplotypes between the localities showed no clear evidence of subdivision in A. opercularis, while in M. varia the results indicated that the two localities analysed should be managed as separate stocks.
Keywords:16S rRNA  12S rRNA  PCR-RFLPs  population structure              Aequipecten opercularis                        Mimachlamys (Chlamys) varia
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